mouse anti Rat CD161:Preservative Free
货号：MCA1427XZ
规格：1 mg
价格：6401
产品类型：流式抗体
品牌：Bio-Rad
抗原： CD161
物种：大鼠
宿主：小鼠
抗体亚型：IgG1
克隆号： 10/78
荧光染料：Preservative Free
| 产品介绍 | | |
| Mouse anti Rat CD161 antibody, clone 10/78recognizes the rat Killer cell lectin-like receptor subfamily B protein, also known as NKR-PI or CD161. CD161 is a 233 amino acid ~60 kDa type II single pass protein containing a singleC-type lectindomain. CD161 is expressed on rat NK cells and T cell subpopulations. CD161 exists in 2 forms NKR-PIa and NKR-PIb, Mouse anti Rat CD161 antibody, clone 10/78 recognizes both forms of CD161 (Liet al.2003). Clone 10/78 competes with another anti CD161 clone, 3.2.3 for binding to antigen.Mouse anti Rat CD161 antibody, clone 10/78 has been successfully employed for thein vivodepletion of rat NK cells in an experimental obesity model (Wrannet al.2010). |
| 产品详情 | | |
Target Species | Rat |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | None present |
| Carrier Free | Yes |
| Immunogen | Purified splenic NK cells from the LEW rat strain. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.Ag8653 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Store at -20°C only.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/50 | 1/100 |
| Functional Assays | √ | | |
| Immunohistology - Frozen | √ | | |
| Immunoprecipitation | √ | | |
| Radioimmunoassays | √ | | |
| Western Blotting | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 1 x106cells in 100ul. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:Preservative Free | MCA1209XZ | F | 1 mg |
| 文献 |
| 1. Gondaira, S.et al.(2020) Immunosuppression in Cows following Intramammary Infusion ofMycoplasma bovis.Infect Immun. 88 (3) Feb 20 [Epub ahead of print].2. Khosa, S.et al.(2020) Bovine Adenovirus-3 Tropism for Bovine Leukocyte Sub-Populations.Viruses. 12 (12) Dec 12 [Epub ahead of print].3. Ramm, R.et al.(2020) Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovinein vivomodel.Xenotransplantation. 27 (2): e12571.4. Ramos, A.et al.(2018) Melatonin enhances responsiveness to Dichelobacter nodosus vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.Vet Immunol Immunopathol. 206: 1-8.5. Okino, C.H.et al.(2020) A polymorphic CD4 epitope related to increased susceptibility toBabesia bovis.in Canchim calves.Vet Immunol Immunopathol. 230: 110132.6. Park, D.S.et al.(2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.Cell Stress Chaperones. 26 (4): 705-20.7. Radley, G.et al.(2020)In Vitro.Benchmarking Study of Ventricular Assist Devices in Current Clinical Use.J Card Fail. 26 (1): 70-79.8. Colombatti Olivieri, M.A.et al.(2021) Evaluation of a virulent strain ofMycobacterium avium.subsp.paratuberculosis.used as a heat-killed vaccine.Vaccine. NNov 10;S0264-410X(21)01433-X.9. Casaro, S.et al.(2022) Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytesVet Immunol Immunopathol. 248: 110417. |
技术参数 产品应用 IHC;IP;Flow;FN;WB
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