| 产品介绍 | | |
| Mouse anti Cat CD4 antibody, clone vpg34recognizes the feline homolog of the human CD4 antigen. CD4 is not expressed on feline monocytes. |
| 产品详情 | | |
Target Species | Cat |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin |
| Immunogen | Immunoaffinity purified feline CD4. |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Fusion Partners | Spleen cells from immunised BALB/c were fused with cells of the NS0 mouse myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
FITC | 490 | 525 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106lymphocytes in 100ul. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
| Mouse IgG1 Negative Control:FITC | MCA1209F | F | 0.1 mg |
| 文献 |
| 1. Webb, C.et al.(2008) Oxidative stress during acute FIV infection in cats.Vet Immunol Immunopathol. 122 (1-2): 16-24.2. Avery, P.R.et al.(2007) Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures.Vet Immunol Immunopathol. 117 (3-4): 222-35.3. McLuckie, A.J.et al.(2016) Detection ofFelis catusgammaherpesvirus 1 (FcaGHV1) in peripheral blood B- and T-lymphocytes in asymptomatic, naturally-infected domestic cats.Virology. 497: 211-6.4. Veir, J.K.et al.(2006) Evaluation of a novel immunotherapy for treatment of chronic rhinitis in cats.J Feline Med Surg. 8 (6): 400-11.5. Sugiarto, S.et al.(2016) Passive immunization does not provide protection against experimental infection withMycoplasma haemofelis.Vet Res. 47 (1): 79.6. Baumann Jet al.(2015) Lack of cross-protection againstMycoplasma haemofelisinfection and signs of enhancement in "Candidatus Mycoplasma turicensis"-recovered cats.Vet Res. 46: 104.7. MirandaL, H.M.et al.(2018) Co-infection with feline retrovirus is related to changes in immunological parameters of cats with sporotrichosis.PLoS One. 13 (11): e0207644.8. Maeta, N.et al.(2019) Lymphokine-activated killer cell transplantation after anti-cancer treatment in two aged cats.Open Vet J. 9 (2): 147-50.9. Sieg, M.et al.(2019) A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood.Viruses. 11 (2) Feb 09 [Epub ahead of print].10. de Oliveira Matheus, L.F.et al.(2021) Effects ofSaccharomyces cerevisiae.cell wall addition on feed digestibility, fecal fermentation and microbiota and immunological parameters in adult cats.BMC Vet Res. 17 (1): 351. |
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