| 产品介绍 | | |
| Mouse anti Human CD88 antibody, clone S5/1recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21).Clone S5/1 has been shown to inhibit the binding of C5a to its receptor. |
| 产品详情 | | |
Target Species | Human |
Species Cross-Reactivity | Target Species | Cross Reactivity |
| Rabbit | √ |
| Mustelid | Expected from Sequence |
| Bovine | √ |
| Ferret | √ |
| Mink | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | Pack Size: 0.2 mg 0.09% Sodium Azide (NaN3) Pack Size: 25 µg 0.09% Sodium Azide (NaN3) |
| Immunogen | C5aR - peptide: Met1 - Asn31. |
| Carrier Free | Yes |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/50 | 1/100 |
| Functional Assays1 | √ | | |
| Immunohistology - Frozen | X | | |
| Immunohistology - Paraffin | √ | | |
| Immunoprecipitation | X | | |
| Western Blotting | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Bio-Rad recommend the use ofMCA1283ELfor functional studies.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 5 x 105cells in 100ul. |
| 数据 |
| Mouse anti Human CD88 antibody, clone S5/1(MCA1283) used to stain formalin fixed, paraffin embedded human spleen sections followed by biotinylated anti Mouse IgG and streptavidin alkaline phosphatase (red). Nuclei were counterstained with hematoxylin (blue). |
| 同型 |
| Description | Product Code | Applications | Pack Size |
Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
| 文献 |
| 1. Schreiber, A.et al.(2009) C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis.J Am Soc Nephrol. 20: 289-98.2. Eglite, S.et al.(2000) Requirements for C5a receptor-mediated IL-4 and IL-13 production and leukotriene C4 generation in human basophils.J Immunol. 165: 2183-9.3. Conroy, A.et al.(2009) C5a enhances dysregulated inflammatory and angiogenic responses to malaria in vitro: potential implications for placental malaria.PLoS One. 4: e4953.4. Kraft, K.et al.(2001) Characterization of sequence determinants within the carboxyl-terminal domain of chemokine receptor CCR5 that regulate signaling and receptor internalization.J Biol Chem. 276: 34408-18.5. Fukuoka, Y.et al.(2008) Generation of anaphylatoxins by human beta-tryptase from C3, C4, and C5.J Immunol. 180: 6307-16.6. Bettoni, S.et al.(2017) Interaction between Multimeric von Willebrand Factor and Complement: A Fresh Look to the Pathophysiology of Microvascular Thrombosis.J Immunol. 199 (3): 1021-40.7. Huang, L.et al.(2005) Discovery of human antibodies against the C5aR target using phage display technology.J Mol Recognit.18: 327-33.8. Tseng CWet al.(2015) Increased Susceptibility of Humanized NSG Mice to Panton-Valentine Leukocidin andStaphylococcus aureusSkin Infection.PLoS Pathog. 11 (11): e1005292. |
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