Pyroptosis 660 Caspase-1 Kit
货号：ICT9158
规格：25 Tests
价格：6605
产品类型：细胞凋亡检测
品牌：Bio-Rad
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| 产品介绍 | | |
| Pyroptosis 660 Caspase-1 Kitutilizes the popular FLICA technology to detect caspase-1 activation. This kits contain the caspase-1 inhibitor reagent YVAD-FMK, which has the preferred binding sequence for caspase-1, Tyr-Val-Ala-Asp (YVAD) (Chapman, 1992). This preferred caspase-1 binding sequence is labeled with 660 a far red fluorescent dye and linked to a fluoromethyl ketone (FMK) reactive entity. Caspase-1 will not cleave the FLICA inhibitor probe; instead, it forms an irreversible covalent bond with the FMK group on the reagent and becomes inhibited from further enzymatic activity. |
| 产品详情 | | |
| Reagents in the Kit | 1 vial of 660-YVAD-FMK caspase-1 inhibitor - lyophilized1 vial Nigericin10x Cellular Wash Buffer, 15 mLFixative, 6 mL1 vial Hoechst Stain, 1 ml |
| Test Principle | To use FLICA, add directly to the cell culture medium, incubate, and wash. FLICA is cell-permeant and will efficiently diffuse in and out of all cells. If there is an active caspase-1 enzyme inside the cell, it will covalently bind with YVAD-FMK and retain the fluorescent signal within the cell. Unbound FLICA will diffuse out of the cell during the subsequent wash steps. Therefore, positive cells will retain a higher concentration of FLICA and fluoresce brighter than negative cells. There is no interference from pro-caspases or inactive forms of the enzymes. After labeling with FLICA, cells can be counter-stained with other reagents and fixed or frozen. Cells labeled with YVAD-FMK can be counter-stained with reagents such as the red live/dead stains Propidium Iodide and 7-AAD. Nuclear morphology may be concurrently observed using Hoechst 33342 (included in the kit), a blue DNA-binding dye. Cells can be viewed through a fluorescence microscope or flow cytometer. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FLICA 660 | 660 | 685 |
| Acknowledgements | FLICA is a trademark of Immunochemistry Technologies, LLC. |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| MULTIPLE STORAGE CONDITIONS APPLY ON ARRIVAL. Store the unopened kit (and each unopened component) according to the storage instructions on each component label. Store the Nigericin at -20℃. Once reconstituted, the Nigericin stock should be used immediately or alliquoted and stored at -20℃ for 12 months. Avoid repeated freezing and thawing. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | Refer to Instructions for Use |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
技术参数 产品类型 flow；if
推荐用于 细胞凋亡检测
组成成分 flow；if
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