Human Anti-Human IgG1 Fc Antibody clone AbD27686
货号：HCA285
规格：0.1 mg
价格：2023
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：IgG1 Fc
物种：其它
宿主：人
抗体亚型：其它
克隆号：AbD27686
荧光染料：Purified
| 产品介绍 | | |
| Human Anti-Human IgG1 Fc Antibody clone AbD27686is a recombinant monovalent Fab antibody, that recognizes human IgG1 Fc, with an affinity of 76 nM. It has been shown to have a greatly reduced affinity (89-fold) for IgG from cynomolgus macaque compared to human IgG1. It also has strongly reduced binding to rhesus macaque IgG. It does not recognize other human immunoglobulin isotypes (IgG2, IgG3, IgG4, IgA, IgE, IgM) or mouse IgG2a.This antibody has been generated by Bio-Rad using the HuCAL® antibody library and CysDisplay® technology. It is sold for research use, with implicit permission for use for the detection of therapeutic antibodies from samples obtained from experimental animals. When tested by Bio-Rad in an antigen capture pharmacokinetic assay format, this antibody performs similarly to Mouse Anti-Human IgG (CH2 Domain) Antibody clone R10Z8E9 (MCA5748G) in 10% cynomolgus macaque serum. It recognizes a different epitope on human IgG1 Fc than clone R10Z8E9. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL PLATINUM®phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.01% Thiomersal |
| Immunogen | Recombinant human IgG1 kappa |
| Approx. Protein ConcePurityntrations | IgG concentration 0.1 mg/ml |
| Affinity | The monovalent intrinsic affinity of this antibody was measured as KD=76 nM by real time, label free molecular interaction analysis on immobilized hIgG1 kappa |
| Acknowledgements | This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.His-tag is a registered trademark of EMD Biosciences. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 产品推荐 |
| Description | Product Code | Applications | Pack Size |
| Hispec Assay Diluent | BUF049A | E | 50 ml |
| Human anti Etanercept | HCA279 | E | 0.1mg |
技术参数 产品应用 elisa
产品类型 单克隆抗体
推荐用于 elisa
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-33967.htm
QQ:1749072012