| 产品介绍 | | |
| Human anti etanercept, clone AbD25939is an antibody that specifically recognizes etanercept as well as the extracellular ligand-binding portion of the 75 kDa (p75) human tumor necrosis factor receptor 2 (TNFR2). Clone AbD25939 does not inhibit the binding of etanercept to its target tumor necrosis factor alpha (TNFα). The antibody binds to an epitope outside the TNFα binding site and can be used to measure the levels of etanercept and biosimilar products (e.g. Etacept, Intacept) in bioanalytical assays.Our anti-etanercept antibodies can be used to develop pharmacokinetic (PK) bridging assays to measure either free drug or total drug. This antibody, in monovalent Fab format, is recommended as the capture antibody. For an assay to detect free drug, use with the inhibitory antibody, clone AbD25940_hIgG4Pro (HCA277P) for detection; for an assay to detect total drug use with p75-Fc-fusion specific clone AbD26183_hIgG1 (HCA279P) for detection.Etanercept (Enbrel®) is a dimeric fusion protein consisting of the extracellular ligand-binding portion of the human 75 kDa (p75) TNFR2 linked to the Fc portion of human IgG1. The Fc component of etanercept contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It is used to treat autoimmune diseases by interfering with TNF, acting as a TNF inhibitor. It binds to both TNFα and TNFβ (Lymphotoxin A). |
| 产品详情 | | |
| Product Form | A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed inE. coli.The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid. |
| Preparation | Metal chelate affinity chromatography |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.01% Thiomersal |
| Immunogen | Etanercept |
| Activity | The monovalent intrinsic affinity of AbD25939 was measured as KD= 3.2 nM by real time, label free molecular interaction analysis on immobilized etanercept. |
| Purity | Purity was tested by SDS-PAGE and Coomassie-staining of a 2 μg sample. |
| Approx. Protein ConcePurityntrations | Antibody concentration 0.5 mg/ml |
| Acknowledgements | This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.Enbrel® is a registered trademark of Immunex Corporation.His-tag is a registered trademark of EMD Biosciences. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 产品推荐 |
| Description | Product Code | Applications | Pack Size |
| Hispec Assay Diluent | BUF049A | E | 50 ml |
| Human anti Etanercept | HCA279 | E | 0.1mg |
| 文献 |
| 1.Harth, S.et al.(2010) A selection fit mechanism in BMP receptor IA as a possible source for BMP ligand-receptor promiscuity.PLoS One. 5(9). pii: e13049.2.Harth, S.et al.(2010) Crystallization of BMP receptor type IA bound to the antibody Fab fragment AbD1556.Acta Crystallogr Sect F Struct Biol Cryst Commun. 66: 964-8. |
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