Rabbit anti AKT1 (pTyr315)/AKT2 (pTyr316)/AKT3 (pTyr312) antibody
货号:AHP2547
规格:50 µl
价格:3613
产品类型:免疫组化一抗
品牌:Bio-Rad
物种:其它
宿主:兔
抗体亚型:IgG
荧光染料:Purified
产品介绍 | | |
Rabbit anti AKT1 (pTyr315)/AKT2 (pTyr316)/AKT3 (pTyr312) antibodyrecognizes AKT1 when phosphorylated at tyrosine 315, AKT2 when phosphorylated at tyrosine 316 and AKT3 when phosphorylated at tyrosine 312. AKT 1, 2 and 3 are serine/threonine protein kinases that play a key role in the PI3K-Akt signaling pathway, known to regulate cell growth, proliferation and survival. AKT1 is phosphorylated at tyrosine 315 (corresponding to tyrosine 316/312 of AKT2/AKT3 respectively) by Src and PTK6, tyrosine phosphorylation is important for growth factor mediated activation of AKT (Zhenget al.2010). |
产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to AKT1 (pTyr315)/AKT2 (pTyr316)/AKT3 (pTyr312) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide50% Glycerol |
Immunogen | Phospho specific-peptide corresponding to residues surrounding tyrosine 315 of human AKT1/ tyrosine 316 of human AKT2/ tyrosine 312 of human AKT3 |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
Store at -20℃Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | 1/500 | 1/1000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
二抗 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
技术参数 产品应用 wb
产品类型 Polyclonal Antibody
推荐用于 wb
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