产品介绍 |
| Rabbit anti BAD antibodyrecognizes human Bcl2-associated agonist of cell death (BAD), also known as Bcl-xL/Bcl-2-associated death promoter, Bcl-2 binding component 6 or Bcl2-L-8. Similar to the other Bcl-2 protein family members, Bax, Bak, Bid and Bock, BAD has a pro-apoptotic function in the apoptosis signaling cascade.The precise role of BAD in apoptosis signaling depends on BAD’s post-translational modification status. Nonphosphorylated BAD forms heterodimers with the two anti-apoptotic Bcl-2 protein family members, Bcl-xL and Bcl-2 (Zhaet al.1996). The binding of BAD to Bcl-xL promotes apoptosis by inhibiting the apoptosis repressor function of Bcl-xL (Yanget al.1995). Once serine phosphorylated, BAD is unable to heterodimerize with Bcl-xL and interacts with 14-3-3 proteins instead (Masterset al.1995). This binding retains BAD in the cytoplasm and thereby enables Bcl-xL to suppress apoptosis (Zhaet al.1996). |
产品详情 |
Target Species | Human |
Species Cross-Reactivity | Target Species Cross Reactivity |
| Mouse Expected from Sequence |
| Rat √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to BAD was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide50% Glycerol |
Immunogen | Synthetic human BAD peptide |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| Store at -20℃Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
Immunofluorescence | √ | 1/10 | 1/100 |
Immunohistology - Paraffin | √ | 1/50 | 1/200 |
Western Blotting | √ | 1/500 | 1/2000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- Rabbit anti BAD antibody detects a band of approximately 18 kDa in PC3 cell lysate under reducing conditions
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文献 |
| References for BAD antibody1.Zha, J.et al.(1996) Serine phosphorylation of death agonist BAD in response to survival factor results in binding to 14-3-3 not BCL-X(L).Cell 87 (4): 619-28.2.Yang, E.et al.(1995) Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death.Cell 80 (2): 285-91.3.Masters, S.C.et al.(2001) 14-3-3 inhibits Bad-induced cell death through interaction with serine-136.Mol. Pharmacol. 60 (6): 1325-31. |
QQ:1749072012