产品介绍 |
| Rabbit anti Human interleukin-1 receptor antagonist antibodyrecognises human Interleukin-1 receptor antagonist (IL-1RA), a naturally occurring inflammatory inhibitor protein, produced by monocytes, macrophages, neutrophils, corneal epithelial cells and fibroblasts, which inhibits the activity of IL-1 alpha and IL-1 beta, by competitively blocking binding to cell surface receptors for IL-1.Genetic variations in IL-1RA gene (IL1RN) are associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4), and IL-1RA has therapeutic applications in rheumatoid arthritis, asthma, psoriasis, sepsis, chronic myelogenous leukaemia and inflammatory bowel disease. |
产品详情 |
Target Species | Human |
Product Form | Purified IgG - lyophilized |
Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide |
AntiserumPreparation | Antiserum to human IL-1RA were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | None present |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
Carrier Free | Yes |
Immunogen | Recombinant human IL-1RA (PHP080) |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml after reconstitution |
Regulatory | For research purposes only |
Guarantee | 6 months from date of despatch |
存储条件 |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.5 | 2.0ug/ml |
Functional Assays | √ | 2.5 | 3.7ug/ml |
Western Blotting | √ | 0.1 | 0.2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- ELISA
- This purified human IL-1RA antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with abiotinylated human IL-1RA antibody(AHP2184B) as the detection reagent andrecombinant human IL-1RA(PHP080) as the standard.
- Western Blotting
- This purified human IL-1RA antibody may used in Western Blotting applications under either reducing or non-reducing conditions withrecombinant human IL-1RA(PHP080) as the positive control
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可用二抗 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
Sheep anti Rabbit IgG:RPE | STAR35A | | |
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