Rabbit anti ATG12 (Internal Region) antibody
货号：AHP1891
规格：0.1 mg
价格：4692
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：ATG12 (Internal Region)
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Alexa Fluor 488
| 产品介绍 | | |
| Rabbit anti ATG12 (Internal Region) antibodyrecognizes human Autophagy-related protein 12 (ATG12).ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA, inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7.There are at least two isoforms of ATG12. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Mouse | √ |
| Rat | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human ATG12 was raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
Immunogen | Synthetic peptide corresponding to a 15 aa sequence from near the centre of human ATG12. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
| Immunohistology - Paraffin1 | √ | 2.5 ug/ml | |
| Western Blotting | √ | 1.0 ug/ml | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control TissueHuman brain
- Western BlottingAHP1891 detects a band of approximately 40 kDa in mouse heart tissue lysate.
|
| 数据 |
| Western blot analysis of mouse heart lysate probed with Rabbit anti Human ATG12 (AHP1891) im the absence (A) or presence (B) of blocking peptide.Immunohistochemical staining of human brain tissue with Rabbit anti Human ATG12 (AHP1891). |
| 相关试剂 |
| Description | Product Code | Applications | Pack Size |
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB | 0.5 mL |
技术参数 产品应用 P*;WB
产品类型 Polyclonal Antibody
推荐用于 P*;WB
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