Rabbit anti AMBRA1 (C-Terminal) antibody
货号：AHP1889
规格：0.1 mg
价格：5160
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：AMBRA1 (C-Terminal)
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti AMBRA1 (C-Terminal) antibodyrecognizes human Activating molecule in beclin-1-regulated autophagy (AMBRA1), also known as WDR94.AMBRA1 is involved in the regulation of autophagy. Research indicates that it controls protein turnover during neuronal development and in regulating normal cell survival and proliferation.At least 5 isoforms of AMBRA have been identified. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Mouse | √ |
| Rat | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human IRGM was raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
Immunogen | Synthetic peptide corresponding to a 15 amino acid sequence from near the carboxy terminus of human AMBRA1. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
| Immunofluorescence | √ | | 20ug/ml |
| Immunohistology - Paraffin1 | √ | 5ug/ml | |
| Western Blotting | √ | 1 | 2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Human brain
|
| 数据 |
| Western blot analysis of whole cell lysate from NIH-3T3 mouse embryonic fibroblast cells probed with Rabbit anti Human AMBRA1 (AHP1889) at 1ug/ml.Immunohistochemical staining of human brain with Rabbit anti Human AMBRA1 (AHP1889) at 5ug/ml |
技术参数 产品应用 IF;P*;WB
产品类型 Polyclonal Antibody
推荐用于 IF;P*;WB
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