| 产品介绍 | | |
| Rabbit anti Human Galectin-3 antibodyrecognizes human Galectin-3 (Gal-3), also known as the Mac-2 antigen. Gal-3 is a 250 amino acid ~35 kDa member of the galectin family of beta-galactoside-binding proteins, which is involved in both intracellular and extracellular functions and exhibits anti-apoptotic properties.Gal-3 is implicated in many biological processes, including cell differentiation, growth, adhesion, angiogenesis and inflammation, and is expressed in a wide range of neoplasms. Gal-3 acts as a regulator of tumor metastasis expression correlates with tumor progression in liver, thyroid, colon, gastric and breast carcinomas (Calificeet al. 2004). |
| 产品详情 | | |
Target Species | Human |
| Western Blotting | AHP1481B may be used under either reducing or non-reducing conditions withPHP188as the positive control. |
| Reconstitution | Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albuminCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. |
Product Form | Purified IgG conjugated to Biotin - lyophilized |
Antiserum Preparation | Antiserum to human galectin-3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 0.1mg/ml |
Immunogen | Recombinant human galectin-3. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Acknowledgements | PrecisionAb is a trademark of Bio-Rad Laboratories |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.25 | 1.0ug/ml |
Western Blotting | √ | 0.1 | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Clickhereto learn how we validate our PrecisionAb range.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 文献 |
| References for Galectin-3 antibody1. Chen, Y.X.et al.(2015) The Sirt1 activator SRT1720 attenuates angiotensin II-induced atherosclerosis in apoE⁻/⁻ mice through inhibiting vascular inflammatory response.Biochem Biophys Res Commun. 465 (4): 732-8.Further Reading1. Dumic, J.et al.(2006) Galectin-3: an open-ended story.Biochim Biophys Acta. 1760 (4): 616-35. |
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