| 产品介绍 | | |
| Rabbit anti BMI-1 antibodyrecognizes 37 kDa polycomb complex protein BMI-1, involved in segment specification, cell growth and maintenance, transcriptional regulation and chromatin modification. BMI-1 is a proto-oncogene which cooperates with the myc oncogene to produce B-lymphomas. It is required in the regulation of normal adult and leukaemic stem cells, making BMI-1 a potential target for stem cell proliferation and anti-cancer therapies. BMI-1 represses transcription at the ink4 locus which contains tumour suppressors p16 and p19Arf.BMI-1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex which ubiquitinates histone-2A at lys119, resulting in a heritable change in the ability of chromatin to express genes. BMI-1 is also required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2 within the PRC1 complex.The Notch ligand DLL4 (delta-like protein 4) enhances T cell proliferation, by directly influencing BMI-1 transcription. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to human BMI-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | 15 amino acid peptide sequence near the centre of human BMI-1. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Frozen | √ | | |
Western Blotting | √ | 0.5 | 1.0ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 数据 |
| Immunocytochemical staining of K562 cells with Rabbit anti Human BMI-1 (AHP1189). |
| 文献 |
| 1. Raaphorst, F.et al. (2003) Self-renewal of hematopoietic and leukemic stem cells: a central role for the Polycomb-group gene Bmi-1.Trends Immunol. 10:522-4.2. Alkema, M.J.et al.(1993) Characterization and chromosomal localization of the human proto-oncogene BMI-1.Hum Mol Genet. 2 (10): 1597-603.3. Lessard, J. & Sauvageau, G. (2003) Bmi-1 determines the proliferative capacity of normal and leukaemic stem cells.Nature. 423 (6937): 255-60. |
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