产品介绍 | | |
Goat anti Horse IgA antibodyrecognizes equine IgA and shows no cross-reactivity with other equine immunoglobulin classes in immunoelectrophoresis. Goat anti Horse IgA antibody may cross-react with IgA from other species. |
产品详情 | | |
Target Species | Horse |
Product Form | Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid |
Antiserum Preparation | Antisera to equine IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography using antigen coupled to agarose beads. |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.05% Proclin 300 0.2% Bovine Serum Albumin |
Immunogen | Purified canine IgA. |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
存储条件 | | |
Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/10000 | 1/100000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
文献 |
1. Lewis, M.J.et al.(2010) IgA in the horse: cloning of equine polymeric Ig receptor and J chain and characterization of recombinant forms of equine IgA.Mucosal Immunol. 3: 610-21.2. Keggan, A.et al.(2013) Production of seven monoclonal equine immunoglobulins isotyped by multiplex analysis.Vet Immunol Immunopathol. 153 (3-4): 187-93.3. van der Kolk, J.H.et al.(2012) Gluten-dependent antibodies in horses with inflammatory small bowel disease (ISBD).Vet Q. 32: 3-11.4. Palm, A.E.et al.(2016) Secretory immunoglobulin A and immunoglobulin G in horse saliva.Vet Immunol Immunopathol. 180: 59-65.5. Pawlas-Opiela, M.et al.(2022) The relationship between the intensity ofGasterophilus intestinalislarvae infection and the serum and salivary humoral immune response in horses.Sci Rep. 12 (1): 17573. |