Goat anti Chicken IgG (Fc):FITC
货号:AAI29F
规格:1 mg
价格:2550
产品类型:荧光二抗
品牌:Bio-Rad
抗原: IgG (Fc)
物种:Chicken
宿主:山羊
抗体亚型:IgG
荧光染料:FITC
产品介绍 | | |
Goat anti Chicken IgG (Fc) antibodyrecognizes the heavy chains of chicken IgG, specifically, epitopes within the Fc region and shows no cross-reactivity with other chicken immunoglobulin classes as assessed by immunoelectrophoresis. This Goat anti Chicken IgG polyclonal antibody does not react with the light chains of chicken IgG.Goat anti chicken IgG (Fc) has been used successfully for the evaluation of circulating levels of IgG in chickens using ELISA in a number of experimental and field situations. |
产品详情 | | |
Target Species | Chicken |
Product Form | Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
Antiserum Preparation | Antisera to chicken IgG were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography. |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) 0.2% Bovine Serum Albumin |
Immunogen | Purified chicken IgG (Fc) fragment. |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
FITC | 490 | 525 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
存储条件 | | |
Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
文献 |
1.Ferdushy, T.et al.(2014) Acquisition of resistance after continuous infection withAscaridia galli.in chickens.Parasitology. : 1-8.2.Radomska KAet al.(2016) Chicken Immune Response afterIn OvoImmunization with Chimeric TLR5 Activating Flagellin ofCampylobacter jejuni.PLoS One. 11 (10): e0164837.3. Vaezirad, M.M.et al.(2018) Chicken immune response following in ovo delivery of bacterial flagellin.Vaccine. Mar 09 [Epub ahead of print].4. Al-karagoly, H.et al.(2019) Turkey humoral and cell-mediated immune responses to a Newcastle viscerotropic vaccine and its association with major histocompatibility complex.Bulg J Vet Med. 22 (1): 26-40.5. Ranchod, H.et al.(2018) The antigenicity and cholesteroid nature of mycolic acids determined by recombinant chicken antibodies.PLoS One. 13 (8): e0200298.6. Naghizadeh, M.et al.(2019) Rapid whole blood assay using flow cytometry for measuring phagocytic activity of chicken leukocytes.Vet Immunol Immunopathol. 207: 53-61.7. Tang, B.et al.(2020) GtxA is a virulence factor that promotes a Th2-like response duringGallibacterium anatis.infection in laying hens.Vet Res. 51 (1): 40. |
技术参数 产品应用 Flow
产品类型 Polyclonal Antibody
推荐用于 Flow
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