CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), FITC, eBioscience™

货号：11-9987-82,11-9987-80

规格：100 µg,25 µg

价格：2167,822

产品类型：流式抗体

品牌：eBioscience

抗原：CD235a

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：HIR2 (GA-R2)

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), FITC, eBioscience™
浓度	0.5 mg/mL	用法：	0.03 µg/test(Flow)

Product Specific InformationDescription: The HIR2 monoclonal antibody reacts with human glycophorin A, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells.Applications Reported: The HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.Applications Tested: The HIR2 (also GA-R2) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. Binding of this antibody to red cells at high antibody concentration causes cell agglutination. This can be used at less than or equal to 0.03 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationGlycophorins A (GPA) and B (GPB) which are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD235a (Glycophorin A) Antibody (11-9987-82) in FlowStaining of normal human whole blood with Mouse IgG2b kappa Isotype Control FITC (Product # 11-4732-42) (blue histogram) or Anti-Human CD235a (Glycophorin a) FITC (purple histogram). Cells in the erythrocyte gate were used for analysis.

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参考文献：

1.Molecular cancer therapeuticsAcquired Resistance to FGFR Inhibitor in Diffuse-Type Gastric Cancer through an AKT-Independent PKC-Mediated Phosphorylation of GSK3β.AuthorsLau WM,Teng E,Huang KK,Tan JW,Das K,Zang Z,Chia T,Teh M,Kono K,Yong WP,Shabbir A,Tay A,Phua NS,Tan P,Chan SL,So JBY2.Stem cells (Dayton, Ohio)Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells."11-9987 was used in Flow cytometry/Cell sorting to investigate the role of KLF1 in maturation of red blood cells."AuthorsYang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM3.Nature communicationsAllele-specific analysis of DNA replication origins in mammalian cells."11-9987 was used in Flow cytometry/Cell sorting to delineate the mechanisms that control the location and timing of firing of replication origins."AuthorsBartholdy B,Mukhopadhyay R,Lajugie J,Aladjem MI,Bouhassira EE4.Scientific reportsCutting-edge analysis of extracellular microparticles using ImageStream(X) imaging flow cytometry."11-9987 was used in Immunocytochemistry to investigate methods to quantify microparticle levels."AuthorsHeadland SE,Jones HR,D'Sa AS,Perretti M,Norling LV5.PloS oneErythroid-specific expression of β-globin from Sleeping Beauty-transduced human hematopoietic progenitor cells."11-9987 was used in Flow cytometry/Cell sorting to investigate methods for efficient delivery of tightly regulated and stably expressed gene products, showing erythroid-specific expression of β-globin using the Sleeping Beauty transposon system."AuthorsSjeklocha LM,Park CW,Wong PY,Roney MJ,Belcher JD,Kaufman DS,Vercellotti GM,Hebbel RP,Steer CJ

技术参数

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