Goat anti Human TRIM5 Alpha (N-Terminal)
货号：AHP1582
规格：0.1 mg
价格：5083
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：TRIM5 Alpha
物种：人
宿主：山羊
抗体亚型：IgG
荧光染料：Purified
产品介绍 |
| Goat anti Human TRIM5 alpha antibodyrecognizes an epitope within the N-terminal (NT) region of human TRIM5alpha, otherwise known as RNF88 (RING finger protein 88), a cytoplasmic protein and member of the TRIM/RBCC family, known to play a vital role in species-specific early defence against retrovirus infection.The association of TRIM5 with specific retroviral capsid sequences determines the effectiveness of the defence against infection. For example, in Old World monkeys, TRIM5alpha specifically associates with HIV type 1 (HIV-1), whereas in humans TRIM5alpha is not effective against HIV-1, but can inhibit strains of the murine leukaemia virus (MLV). |
产品详情 |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human TRIM5alpha (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin |
Immunogen | Peptide sequence ASGILVNVKEEVTC corresponding to the N-Terminal region of TRIM5 (NP_149023.1; NP_149083.1; NP_149084.1). |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/16000 |
Immunohistology - Paraffin1 | √ | | 1.0ug/ml |
Western Blotting | √ | 1.0 | 3.0ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Human tonsil
- Western Blotting
- AHP1582 detects a band of approximately 37kDa in human brain cell lysates.
|
可用二抗 |
Description | Product Code | Applications | Pack Size |
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
相关产品 |
Description | Product Code | Applications | Pack Size |
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml |
文献 |
| 1.Lee, K. & Kewalramani, V.N. (2004) In defense of the cell: TRIM5alpha interception of mammalian retroviruses.Proc Natl Acad Sci U S A. 101 (29): 10496-7. |
技术参数 产品应用 E ;P* ;WB
产品类型 Polyclonal Antibody
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