CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), PerCP-eFluor 710
货号：46-2799-41,46-2799-42
规格：25 Tests,100 Tests
价格：1340,3402
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
荧光染料：PerCP-eFluor™ 710
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor 710 |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.25 μg)/test (Flow) |
| 产品详细信息Description: The J105 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells.Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (cat. 11-9969).Applications Reported: This eBioJ105 (J105) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioJ105 (J105) antibody has been pre-titrated and tested by flow cytometric analysis of activated human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
| 数据 |
| CD279 (PD-1) Antibody (46-2799-41) in FlowStaining of unstimulated (blue histogram) or 3-day PHA-stimulated (purple histogram) normal human peripheral blood cells with Anti-Human CD279 (PD-1) PerCP-eFluor® 710. Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Journal of virologyDynamics of memory B-cell populations in blood, lymph nodes, and bone marrow during antiretroviral therapy and envelope boosting in simian immunodeficiency virus SIVmac251-infected rhesus macaques."46-2799 was used in Flow cytometry/Cell sorting to investigate memory B-cell subpopulations and plasma cells/plasmablasts of rhesus macaques during, and upon release of, antiretoviral treatment, showing that long-term memory does not form."2.BMC complementary and alternative medicineRandomized pilot trial of a synbiotic dietary supplement in chronic HIV-1 infection."46-2799 was used in Flow cytometry/Cell sorting to study the effect of a synbiotic dietary supplement in the augmentation of probiotic species in the gut during chronic HIV-1 infection."3.Journal of immunology (Baltimore, Md. : 1950)Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques."46-2799 was used in Flow cytometry/Cell sorting to quantify SIV Env-specific IL-21-producing T follicular helper cells in response to vaccination."4.Journal of immunology (Baltimore, Md. : 1950)Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques."46-2799 was used in Flow cytometry/Cell sorting to quantify SIV Env-specific IL-21-producing T follicular helper cells in response to vaccination."5.Frontiers in immunologyInhibitory Receptor Expression Depends More Dominantly on Differentiation and Activation than "Exhaustion" of Human CD8 T Cells."46-2799 was used in Flow cytometry/Cell sorting to evaluate the direct relationship between inhibitory receptor expression and functionality in CD8 T cells." |
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