产品介绍 |
| Guinea pig anti Pig insulin antibodyrecognizes porcine insulin, a hormone secreted by the beta cells of the islets of Langerhans in the pancreas. Insulin belongs to a family of hormones including the insulin-like growth hormones and the relaxins. The insulin precursor is a 108 amino acid polypeptide consisting of a 24 aa signal peptide, an insulin A chain, a prohormone polypeptide (also known as the ‘C chain’) and an insulin B chain together having a predicted molecular mass of 11,672 Da. Active insulin migrates with an apparent molecular mass of ~6 kDa in western blotting of porcine, bovine and lapine samples (Schechteret al.1992).Insulin is a hormone that functions in carbohydrate homeostasis, and acts to decrease blood glucose concentration by increasing cell permeability to monosaccharides, amino acids and fatty acids, and accelerate glycolysis and glycogen synthesis in the liver, it induces uptake of glucose into cells through its interaction with the glucose transporter GLUT4 (Watson and Pressin 2001). Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumours of B-cell origin such as insulinoma (Schielet al.2006).Guinea pig anti Pig insulin antibody has been successfully used to demonstrate the presence of insulin in the islets of Langerhans of murine pancreas using immunohistochemistry on paraffin embedded material (Ortsäteret al.2012). |
产品详情 |
Target Species | Pig |
Species Cross-Reactivity | Target Species | Cross Reactivity |
| Mouse | √ |
| Human | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G |
Buffer Solution | Phosphate buffered saline. |
Preservative Stabilisers | 0.09%Sodium Azide (NaN3) |
Immunogen | Native. |
Approx. Protein Concentrations | IgG concentration 5.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
Immunohistology -Frozen | √ | 1/50 | 1/1600 |
Immunohistology - Paraffin | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Pancreas.
|
可用二抗 |
Description | Product Code | Applications | Pack Size |
Goat anti Guinea Pig IgG (H/L):HRP | AHP863P | C E P | 1 mg |
相关产品 |
Description | Product Code | Applications | Pack Size |
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml |
文献 |
| 1.Ortsäter, H.et al.(2012) Diet supplementation with green tea extract epigallocatechin gallate prevents progression to glucose intolerance in db/db mice.Nutr Metab (Lond). 9: 11.2.Izumi, K.et al.(2015) Reduced Tyk2 gene expression in β-cells due to natural mutation determines susceptibility to virus-induced diabetes.Nat Commun. 6: 6748.3.Tian, J.et al.(2019) A Clinically Applicable Positive Allosteric Modulator of GABA Receptors Promotes Human β-Cell Replication and Survival as well as GABA’s Ability to Inhibit Inflammatory T CellsJ Diabet Res. 2019, Article ID 5783545, 7 pages. |
QQ:1749072012