LavaLAMP™ RNA Master Mix
货号：30086-1,30087-1
规格：200 rxn ,with Dye，200 rxn
价格：5051,5231
产品类型：PCR 及DNA聚合酶
品牌：Lucigen
| The LavaLAMP™ RNA Master Mix is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. These enzyme features make it possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) in which the RNA target is first converted into cDNA and then that cDNA acts as template for additional LAMP, all using a single DNA polymerase. By combining the strong activity of this enzyme with an optimized master mix formulation, we’ve produced an easy-to-use system that enables fast, sensitive detection of specific RNA targets. |
| 优点： |
| - Isothermal Amplification:Facilitates running amplification reactions with simplified instrumentation；-Master Mix Format:Streamlines reaction setup while reducing potential handling errors；-Minimal Optimization:Focuses optimization on the two critical reaction parameters - primer design and reaction temperature |
| 数据： |
| Figure 1. The differences in RNA LAMP reaction set up and potential optimization parameters when using a master mix vs. a component kit format.Panel A illustrates the components added to a RNA LAMP reaction (left) when using the LavaLAMP™ RNA Master Mix and the various parameters than can be optimized (right). Panel B illustrates reaction setup (left) and potential optimization parameters (right) when using a standard RNA LAMP component kit.Bold textindicates components or parameters common to both systems.Figure 2. Loop-mediated isothermal amplification (LAMP) with real-time fluorescent detection of amplified products.RNA LAMP reactions (6 replicates per condition) were set up using the indicated kits according to manufacturer’s recommendations. Target RNA (MS2 bacteriophage) at varying input amounts, MS2-specific RNA LAMP primers, and Green Fluorescent Dye (LavaLAMP™ RNA Master Mix Kit) were included in all reactions. Reactions were run on a CFX96 Thermal Cycler (Bio-Rad) at the following temperatures: LavaLAMP; 68°C; other kits at the recommended 65°C and fluorescence was measured over 60 minutes to determine the TTR. NTC denotes No Target Control.Figure 3: RNA LAMP-based detection of Zika Virus RNA.RNA LAMP reactions (6 replicates per condition) were set up using the indicated kits according to manufacturer’s recommendations. Target Zika virus RNA at varying input amounts, Zika-specific RNA LAMP primers, and Green Fluorescent Dye (LavaLAMP™ RNA Master Mix Kit) were included in all reactions. Reactions were run on a CFX96 Thermal Cycler (Bio-Rad) at the following temperatures: LavaLAMP; 68°C; WarmStart® LAMP Kit; 65°C, and fluorescence was measured over 60 minutes to determine the TTR. NTC denotes No Target Control. |
| 组成成分： |
| 30086-1#▪ LavaLAMP™ RNA Master Mix ( F824234-1) 2 x 1.25 mL▪ RNA Positive Control LAMP Primer Mix ( F814233-1) 25 µL▪ RNA Positive Control ( F824232-1) 10 µL30087-1#▪ LavaLAMP™ RNA Master Mix ( F824234-1) 2 x 1.25 mL▪ RNA Positive Control LAMP Primer Mix ( F814233-1) 25 µL▪ RNA Positive Control ( F824232-1) 10 µL▪ Green Fluorescent Dye ( F883827-1) 200 µL |
| 相关产品： |
| ▪Green Fluorescent Dye (#30078-2) |
技术参数 产品优点 - Isothermal Amplification: Facilitates running amplification reactions with simplified instrumentation- Master Mix Format: Streamlines reaction setup while reducing potential handling errors- Minimal Optimization: Focuses optimization on the two critical reaction parameters - primer design and reaction temperature- Lyophilization-ready: Avoids redesign of assays to remove components known to inhibit lyophilization – all components are lyophilization-compatible
产品应用 Sensitive RNA detection with this easy-to-use RNA LAMP (RT-LAMP) Master Mix
组成成分 30086-1#LavaLAMP™ RNA Master Mix ( F824234-1) 2 x 1.25 mLRNA Positive Control LAMP Primer Mix ( F814233-1) 25 µLRNA Positive Control ( F824232-1) 10 µL30087-1#LavaLAMP™ RNA Master Mix ( F824234-1) 2 x 1.25 mLRNA Positive Control LAMP Primer Mix ( F814233-1) 25 µLRNA Positive Control ( F824232-1) 10 µLGreen Fluorescent Dye ( F883827-1) 200 µL
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