| 产品介绍 | | |
| Mouse anti Human GM-CSF antibody, clone 3209recognizes recombinant and native human granulocyte macrophage colony stimulating factor (GM-CSF).Human Granulocyte colony stimulating factor (GM-CSF) is a 14.6 kDa glycoprotein, which is produced by T cells, macrophages, fibroblasts and endothelial cells. GM-CSF is involved in the stimulation of proliferation and differentiation of granulocyte and macrophage progenitor cells.The neutralization dose (ND50) of this batch, as determined by the proliferation of TF-1 cells, is approximately 0.3-0.5 μg/ml in the presence of 0.5 ng/ml of recombinant human GM-CSF. The ND50 may vary depending on conditions. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <5% trehalose |
Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
Immunogen | Recombinant protein corresponding to amino acids 14 to 144 of human GM-CSF produced inE. coli. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20℃only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.5 | 1.0 ug/ml |
Functional Assays | √ | 0.3 | 0.5 ug/ml |
| Immunohistology - Frozen | √ | 8.0 | 25 ug/ml |
Western Blotting1 | √ | 1.0 | 2.0 ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.14740-0955 should be used under non-reducing conditions, reducing conditions are not recommended.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
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