| 产品介绍 | | |
| Rabbit anti Mouse Collagen I antibodyrecognizes murine collagen 1. Collagen 1 is a fibrillar forming collagen, a secreted protein forming part of the extracellular matrix. Less than 1% cross reactivity has been observed with mouse collagen, types II, VI and III and human, chicken and rat collagen, type I. |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified Ig - liquid |
Preparation | Purified Ig prepared by affinity chromatography |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3)Antibiotic antimycotic mixture 1% |
Immunogen | Native type I collagen from mouse skin. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/100 | 1/400 |
Immunofluorescence | √ | 1/50 | 1/100 |
| Western Blotting | √ | | |
| Immunohistology - Paraffin | √ | 1/250 | 1/1000 |
| Radioimmunoassays | √ | | |
Immunohistology - Frozen | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
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| 数据 |
| Published customer image:Rabbit anti Mouse Collagen I antibody (2150-1410) used to demonstrate collagen I expressing fibroblastic reticular cells (FRCs)in vitroby immunofluorescence.Image caption:Confocal images of FRCs treated for 4 days with CCM, B16.F10 TCM, Veh– H2O, or 15 mmol/L LA and stained for collagen I (red) and nuclei (blue; left) and quantification thereof (right). n=2 independent experiments each with 8 fields of view analyzed. Scale bar: 50μm. Data are mean with SEM. Significance (, P <0.05; , P <0.01; , P <0.001; and , P <0.0001) was determined by unpaired two-tailed t test. B16, B16.F10. |
| 文献 |
| 1. Uezumi, A.et al.(2011) Fibrosis and adipogenesis originate from a common mesenchymal progenitor in skeletal muscle.J Cell Sci. 124: 3654-64.2. Takase, H.M.et al.(2013) FGF7 is a functional niche signal required for stimulation of adult liver progenitor cells that support liver regeneration.Genes Dev. 27: 169-81.3. Birbrair Aet al.(2013) Type-1 pericytes participate in fibrous tissue deposition in aged skeletal muscle.Am J Physiol Cell Physiol. 305 (11): C1098-113.4. Randles, M.J.et al.(2015) Genetic Background is a Key Determinant of Glomerular Extracellular Matrix Composition and Organization.J Am Soc Nephrol. 26 (12): 3021-34.5. Ma, M.S.et al.(2016) Characterization and comparison of osteoblasts derived from mouse embryonic stem cells and induced pluripotent stem cells.J Bone Miner Metab. Jan 8. [Epub ahead of print] |
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