| 产品介绍 | | |
| Native Murine collagen Iis purified Mouse collagen I from tail tendon. Thermal denaturation converts the collagen to gelatin.Impurities:Mouse collagen type III 10%Mouse collagen (other types) <1%Non-collagenous proteins <0.5% |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified Protein - liquid |
Preparation | Collagens were extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. Collagen type I was purified by using differential salt precipitation. |
Buffer Solution | 0.5M acetic acid |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | 1.0 mg/ml |
Purity | 90%< by SDS PAGE (cross linked collagen type I dimers and trimers represent ~10%) |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Protein Molecular Weight | ~300 kDa |
| 存储条件 | | |
| Store at -20℃only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1. Rhodes, R.K. & Miller, E.J. (1978) Physicochemical characterization and molecular organization of the collagen A and B chains.Biochemistry. 17 (17): 3442-8.2. Sebinger, D.D.et al.(2013) ECM modulated early kidney development in embryonic organ culture.Biomaterials. 34 (28): 6670-82.3. Takahashi, S.et al.(2015) C-type lectin-like domain and fibronectin-like type II domain of phospholipase A2 receptor 1 modulate binding and migratory responses to collagen.FEBS Lett. 589 (7): 829-35. |
QQ:1749072012