| 产品介绍 | | |
| Human collagen type IV from placenta.Human collagen type I-III, V, VI (<5%). Non collagen proteins (<0.5%). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified protein - liquid |
Preparation | Salt and alcohol precipitations, DEAE chromatography. Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurement, ability to form microfibrils and by reaction with anti-collagen type specific monoclonal antibodies. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Approx. Protein Concentrations | Total protein concentration 1.0 mg/ml |
Purity | >95% by SDS-PAGE |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Protein Molecular Weight | 340 kDa |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 文献 |
| References for Collagen IV1. Takahashi, S.et al.(2015) C-type lectin-like domain and fibronectin-like type II domain of phospholipase A2 receptor 1 modulate binding and migratory responses to collagen.FEBS Lett. 589 (7): 829-35.Further Reading1. Glanville, R.W.et al.(1979) Isolation and characterization of a native placental basement-membrane collagen and its component alpha chains.Eur J Biochem. 95 (2): 383-9.2. Sage, H. & Bornstein, P. (1979) Characterization of a novel collagen chain in human placenta and its relation to AB collagen.Biochemistry. 18 (17): 3815-22.3. Klasson, S.C.et al.(1986) The effects of tissue pretreatment and pepsin levels on the isolation of collagens from human placenta.Coll Relat Res. 6 (5): 397-408.4. Bornstein, P. & Sage, H. (1980) Structurally distinct collagen types.Annu Rev Biochem. 49: 957-1003. |
QQ:1749072012