产品介绍 | | |
| Goat anti Mouse IgG antibodyrecognizes mouse IgG, recognising the heavy chain of mouse IgG1, IgG2a, IgG2b and IgG3 as demonstrated by ELISA.Goat anti Mouse IgG antibody has been cross-adsorbed against mouse IgM, mouse IgA and human serum to reduce potential cross-reactivity. |
产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
Antiserum Preparation | Antisera to mouse IgG were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose. |
Buffer Solution | Phosphate buffered saline |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | |
Immunofluorescence | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
文献 |
| 1.Joimel, U.et al.(2010) Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.BMC Cancer. 10: 375.2.Childs Ket al.(2012) Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction.J Virol. 86 (7): 3411-21.3.Moalli, F.et al.(2015) Intravital and whole-organ imaging reveals capture of melanoma-derived antigen by lymph node subcapsular macrophages leading to widespread deposition on follicular dendritic cells.Front Immunol. 6: 114.4.Ramos-Sevillano, E.et al.(2016) PSGL-1 on Leukocytes is a Critical Component of the Host Immune Response against Invasive Pneumococcal Disease.PLoS Pathog. 12 (3): e1005500.5.Abbate, F.et al.(2016) Acid-sensing ion channel immunoreactivities in the cephalic neuromasts of adult zebrafish.Ann Anat. 207: 27-31.6.Shea, G.K.et al.(2020) Juxtacrine signalling via Notch and ErbB receptors in the switch to fate commitment of bone marrow-derived Schwann cells.Eur J Neurosci. 52 (5): 3306-21. |
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