Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 350/兔抗小鼠IgG (H+L)交叉吸附二抗， Alexa Fluor 350

货号：A-21062

规格：1 mg

价格：3223

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：Alexa Fluor 350

抗体类型:	荧光二抗	同型对照：
浓度：	2 mg/mL	用法：	1-10 µg/mL (Flow/ICC/IHC/IF)

靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

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参考文献：

1.Journal of molecular medicine (Berlin, Germany)EWS/FLI-1 oncoprotein subtypes impose different requirements for transformation and metastatic activity in a murine model.

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