产品介绍 | | |
| Mouse anti Human angiotensin I antibody, clone 13/96/Ang C9recognizes angiotensin I, a 10 amino acid peptide derived by the proteolytic processing of angiotensinogen. Angiotensin I is important in the Renin-Angiotensin-Aldosterone System (RAAS). RAAS is responsible for the regulation of blood pressure and water retention in the body.Angiotensin I is produced from a reaction between the renal enzyme Renin and angiotensinogen. It is further converted by Angiotensin Converting Enzyme (ACE) into a vasoactive form called Angiotensin II which is responsible for increasing blood pressure. Mouse anti Human angiotensin I antibody does not react with angiotensin II, III or angiotensinogen in ELISA. |
产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
Immunogen | HMW conjugate. |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.- ELISA
- In a simple indirect ELISA, using 1.0 µg of Angiotensin I coated per well, the 50% binding was observed at an antibody dilution of 1/950. Under the same conditions, the end point titre was observed at an antibody dilution of 1/10K.
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文献 |
| 1.Luna-Vital, D.A.et al.(2016) Dietary peptides from the non-digestible fraction ofPhaseolus vulgarisL. decrease angiotensin II-dependent proliferation in HCT116 human colorectal cancer cells through the blockade of the renin-angiotensin system.Food Funct. 7 (5): 2409-19. |
QQ:1749072012