| 产品介绍 | | |
| Rabbit anti Human C4d antibodyis specific for human complement split product C4d. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified Ig - liquid |
Preparation | Purified Ig prepared by affinity chromatography on Protein G |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.1% Proclin 950 |
Immunogen | Synthetic peptide corresponding to amino acids 1241-1256 of C4 conjugated to Keyhole limpet Haemocyanin. |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunofluorescence | √ | 1/20 | 1/80 |
| Immunohistology - Paraffin1 | √ | 1/20 | 1/80 |
Immunohistology - Frozen | √ | 1/20 | 1/80 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. TRIS/EDTA buffer pH 8.5 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
Immunohistochemical staining of human kidney with Rabbit anti Human C4d antibody (0300-0230) showing localization to the peritubular capillaries Formalin fixed paraffin embedded human kidney for a patient with Lupus erythematosus stained with Rabbit anti Human C4d antibody (0300-0230) following Sodium citrate (pH6.0) mediated antigen retrieval: high power |
| 文献 |
| 1. Titipungul, T.et al.(2012) Percentage of C4d staining in lupus nephritis and correlation between C4d staining with serum C3, serum C4 level and other parameter in lupus nephritis.Mahasarakham Hospital Journal 9: 27-342. Regele, H.et al.(2001) Endothelial C4d deposition is associated with inferior kidney allograft outcome independently of cellular rejection.Nephrol Dial Transplant. 16 (10): 2058-66.3. Santos, A.et al.(2012) C4d detection in renal allograft biopsies: immunohistochemistry vs. immunofluorescencePort J Nephrol Hypert 26 (4): 272-7.4. Sethi, S.et al.(2015) C4d as a Diagnostic Tool in Proliferative GN.J Am Soc Nephrol. 26 (11): 2852-9.5. Viana, H.et al.(2009) C4d presence in kidney allograft biopsy: sensitivity and specificity of immunoperoxidase vs. immunofluorescence.N: 10th Banff Conference on Allograft Pathology, 2009, 9 a 14 Ago. Banff, Alberta, Canada |
QQ:1749072012