| 产品介绍 | | |
| Mouse anti Human CD21 antibody, clone LB21recognizes the human Complement receptor type 2, also known as CD21 or the Epstein-Barr virus receptor. CD21 is a ~14 kDa cell surface glycoprotein expressed by mature B cells and by follicular dendritic cells. The molecule acts as a receptor for complement components C3d, C3dg and iC3b, as well as for Epstein Barr Virus. It forms part of a large signal transduction complex in association with CD19. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
Sheep | √ |
Mink | √ |
Bovine | √ |
Goat | √ |
Cat | √ |
N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
Reconstitution | Pack Size: 100 Tests |
Reconstitute with 1 ml distilled water |
Pack Size: 25 Tests |
Reconstitute in 0.25 ml disilled water |
Preparation | Purified IgG prepared by ion exchange chromatography |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin |
Immunogen | Human IM9 cell line. |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
RPE 488nm laser | 496 | 578 |
| 存储条件 | | |
| Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | 1/5 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells or 100ul whole blood |
| 同型对照 |
Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
| 相关产品 |
Description | Product Code | Applications | Pack Size |
Human Seroblock | BUF070A | F | 50 Test |
Human Seroblock | BUF070B | F | 200 Test |
| 文献 |
| 1. Moingeon, P.et al.(1989) The structural biology of CD2.Immunol Rev. 111: 111-44.2. Beyers, A.D.et al.(1989) Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen.Immunol Rev. 111: 59-77.3. Lin, C.W.et al.(2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.Blood. 106 (10): 3567-74.4. Kap, Y.S.et al.(2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.J Histochem Cytochem. 57: 1159-67. |
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