| 产品介绍 | | |
| Mouse anti Human CD8 antibody, clone 4B11recognizes the human CD8 cell surface antigen, a ~32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells. Mouse anti Human CD8 antibody, clone 4B11 was raised based on an earlier successful strategy used to generate rabbit polyclonal antibodies against human CD8 employing the same immunizing peptide (Masonet al.1992).Mouse anti Human CD8 antibody, clone 4B11 has been reported as being suitable for use in Western blotting (Williamsonet al.1998) . |
| 产品详情 | | |
Target Species | Human |
| Product Form | Tissue Culture Supernatant - liquid |
Preservative Stabilisers | Pack Size: 1 ml 0.09% Sodium Azide Pack Size: 0.1 ml <0.1% Sodium Azide (NaN3) |
| Immunogen | Synthetic peptide derived from the carboxy terminal region of the human CD8 alpha chain coupled to a N-terminal cysteine, with the sequence C-KSDGKPSLSARYV. The peptide was coupled to bovine serum albumin and keyhole limpet hemocyanin. |
| Fusion Partners | Spleen cells from immunised mice were fused with cells of a mouse p3-NS1-Ag4-1 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Frozen | √ | | 1/50 |
| Immunohistology - Paraffin1 | √ | | 1/50 |
| Western Blotting | √ | | 1/25 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Tris/EDTA buffer pH 8.0 is recommended for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Histology Positive Control TissueHuman Tonsil
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| 数据 |
| Formalin fixed, paraffin processed human tonsil stained with Mouse anti Human CD8 antibody, clone 4B11. (MCA1817) Mag. 40X |
| 文献 |
| 1. Choi, Y.et al. (2009) Immunohistochemical Characterization of the Human Sublingual Mucosa.2. Kim, Y.C.et al.(2010) Presence ofPorphyromonas gingivalisand plasma cell dominance in gingival tissues with periodontitis.Oral Dis. 16: 375-81.3. Ruf, M.T.et al.(2011) Secondary buruli ulcer skin lesions emerging several months after completion of chemotherapy: paradoxical reaction or evidence for immune protection?PLoS Negl Trop Dis. 5: e1252.4. Matsuura, E.et al.(2017) Dynamic acquisition of HTLV-1 tax protein by mononuclear phagocytes: Role in neurologic disease.J Neuroimmunol. 304: 43-50.5. Weber, B.et al.(2017) Distinct interferon-gamma and interleukin-9 expression in cutaneous and oral lichen planus.J Eur Acad Dermatol Venereol. 31 (5): 880-6.6. Choi, Y.S.et al.(2016) The presence of bacteria within tissue provides insights into the pathogenesis of oral lichen planus.Sci Rep. 6: 29186.7. Sabolek, M.T..et al.(2019) Communication of CD8+T cells with mononuclear phagocytes in multiple sclerosis.Ann Clin Transl Neurol. 6 (7): 1151-64.8. Kim, H.D.et al.(2021) Implication of CD69+CD103+tissue-resident-like CD8+T cells as a potential immunotherapeutic target for cholangiocarcinoma.Liver Int. 41 (4): 764-76. |
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