StarBright Blue 700 Goat Anti-Mouse IgG
货号：12004158,12004159
规格：400 µl ,80 µl
价格：1505,595
产品类型：荧光二抗
品牌：Bio-Rad
物种：小鼠
宿主：山羊
抗体亚型：IgG
荧光染料：StarBright Blue 700
产品介绍 | | |
| Use StarBright Blue 700 Goat Anti-Mouse IgG for ultra-sensitive detection of proteins in fluorescence western blotting. This secondary antibody is ideal for use in multiplex blotting, eliminating the need for stripping and reprobing, or cutting blots. StarBright Blue 700 Goat Anti-Mouse IgG is optimized for use with the ChemiDoc MP Imaging System, permitting detection of up to three proteins in a single blot.The StarBright Fluorophore is composed of multiple monomers that each absorb and emit light, giving a higher quantum yield than other fluorophores and creating a very strong signal. Additionally, the large separation between Exmaxand Emmax(470/700 nm) results in minimal spectral overlap between emission and excitation wavelengths.Schematic representation of a StarBright Label conjugated to an antibody.The light-absorbing monomer is excited at 470 nm and, via an efficient intramolecular transfer process, the energy is transmitted to a light-emitting monomer, which emits at 700 nm. An antibody is conjugated to linker monomers. |
优点 | | |
| - Easy multiplexing —can be used with IRDye 800 Antibodies or other common fluorophores to detect multiple proteins on the same blot- Shorter exposure time —the exceptional brightness of the StarBright Fluorophore leads to exposure times orders of magnitude lower than traditional fluorescent dyes: seconds vs. minutes- High signal-to-noise ratio —emits in the infrared range of the spectrum, where there is minimal background (excitation/emission: 470/700 nm)- Low nonspecific binding —conjugated to highly cross-adsorbed antibodies |
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