| 产品介绍 | | |
| StarBright Blue 700 Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. The StarBright Fluorophore is composed of a condensed polymer made up of multiple light-absorbing and -emitting monomers which provides an exceptionally bright signal (high quantum yield) compared to most traditional fluorophores.StarBright Blue 700 Fluorescent Secondary Antibodies can be used with traditional fluorophores like the DyLight 800 Fluorophore for multiplexing. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or the hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot.Features and BenefitsEasy multiplexing— StarBright Secondary Antibodies can be used with IRDye 800 or other common fluorophores to detect multiple proteins on the same blotShort exposure time— the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutesHigh signal-to-noise ratio— StarBright Secondary Antibodies emit in the red/far red, where there is minimal background (Exmax/Emmax= 470 nm/700 nm)Low nonspecific binding— StarBright Fluorophore is conjugated to highly cross-adsorbed antibodiesGoat Anti-Rabbit IgG StarBright Blue 700 has been cross-adsorbed against bovine, goat, human, mouse and rat. |
| 产品详情 | | |
Target Species | Rabbit |
Product Form | Purified IgG conjugated to StarBright Blue 700 - lyophilized |
Reconstitution | Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at max speed in a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube. Do not centrifuge more than 10 sec. |
| Antiserum Preparation | Antiserum to Rabbit IgG was raised by repeated immunisation of Goats with highly purified antigen. |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | >80% Sucrose<1% Polyethylene Glycol<0.04% 2-Methyl-2H-Isothiazol-3-One<10% Bovine Serum Albumin |
Immunogen | Normal human peripheral blood lymphocytes. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
StarBright Blue 700 | 470 | 700 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
| 存储条件 | | |
| 1 year at -20oC lyophilized; 6 months at +4oC after resuspension.This product is photosensitive and should be protected from lightDO NOT FREEZE the solubilized material |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | x | | |
| Western Blotting | √ | 1/2500 | 1/5000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
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| 文献 |
| 1. Li, T.et al.(2021) RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation.Nat Commun. 12 (1): 1055.2. Vasileva, L.V.et al.(2021) Rosmarinic acid attenuates obesity and obesity-related inflammation in human adipocytes.Food Chem Toxicol. 149: 112002.3. Dunbar, K.et al.(2021) IMiDs induce FAM83F degradation via an interaction with CK1α to attenuate Wnt signalling.Life Sci Alliance. 4 (2)Dec 23 [Epub ahead of print].4. Dunbar, K.et al.(2021) FAM83F regulates canonical Wnt signalling through an interaction with CK1&alpha.Life Sci Alliance. 4 (2)Dec 24 [Epub ahead of print].5. Holoch, D.et al.(2021) A cis-acting mechanism mediates transcriptional memory at Polycomb target genes in mammals.Nat Genet. 53 (12): 1686-1697.6. Goad, D.W.et al.(2021) Acquired chemoresistance can lead to increased resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virusMolecular Therapy - Oncolytics. Dec 01 [Epub ahead of print].7. Reisman, B.J.et al.(2021) Apoptolidin family glycomacrolides target leukemia through inhibition of ATP synthase.Nat Chem Biol. Dec 02 [Epub ahead of print].8. Riera-Tur, I.et al.(2022) Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.Life Sci Alliance. 5 (3)Dec 21 [Epub ahead of print].9. Meissner, M.E.et al.(2022) Differential Activity of APOBEC3F, APOBEC3G, and APOBEC3H in the Restriction of HIV-2.J Mol Biol. 434 (2): 167355.10.Nowicka, N.et al.(2022) The Involvement of RAGE and Its Ligands during Progression of ALS in SOD1 G93A Transgenic Mice.Int J Mol Sci. 23 (4): 2184' |
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