| LysoTracker® Deep Red is a deep red-fluorescent dye for labeling and tracking acidic organelles in live cells.The LysoTracker® probes consist of a fluorophore linked to a weak base that is only partially protonated at neutral pH. This allows LysoTracker® probes to freely permeate cell membranes enabling them to label live cells. LysoTracker® probes are highly selective for acidic organelles, and unlike dinitrophenyl (DNP), LysoTracker® probes do not require use of a second antibody for detection.LysoTracker® probes are available in a variety of fluorescent colors. |
| 优点： |
| - High selectivity for acidic organelles- Effective labeling of live cells at nanomolar concentrations- Live-cell imaging- With excitation/emission of 647/668 nm, the same filter set can be used as used for Cy5 or MitoTracker® Deep Red- Simple, Highly Specific, One-Step Staining and Tracking of Acidic Organelles |
| 数据： |
| Co-localization while using LysoTracker® Deep Red.HeLa cells expressing CellLight® Peroxisome-RFP was loaded with 50nM MitoTracker® Green and 50nM LysoTracker® Deep Red. Images were pseudo colored as LysoTracker® Deep Red (Blue), MitoTracker® Green (green) and CellLight® Peroxisome-RFP (Red).LysoTracker® Deep Red labels lysosomes.Top Panel: HeLa cells expressing CellLight® Lysosomes-GFP (green) were labeled with 50nM LysoTracker® Deep Red (red) and imaged on a Zeiss LSM confocal microscope. The pattern of LysoTracker® Deep Red labeling co- localizes with that of a lysosomal targeted GFP. Bottom panel. LysoTracker® Deep Red is contained within the lumen, not the membrane, of the lysosome. U-2 )S cells expressing CellLight® Lysosomes-GFP (green) were labeled with 50nM LysoTracker® Deep Red (red) and imaged using a Zeiss LSM confocal microscope. An area of the cell on the left is magnified and form these images it is possible to see that that the LysoTracker® Deep Red is contained within the lumen of the lysosome and doesn’t label the lysosomal membrane. |
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