| Description: The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express this molecule. CD19, along with CD21, CD81, Leu13, and MHC class II, form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.Applications Reported: This SJ25C1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SJ25C1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1.eLifeMalaria-associated atypical memory B cells exhibit markedly reduced B cell receptor signaling and effector function.2015"45-0198 was used in Flow cytometry/Cell sorting to elucidate the function of atypical memory B cells and their relationship to classical memory B cells."Authors Portugal S,Tipton CM,Sohn H,Kone Y,Wang J,Li S,Skinner J,Virtaneva K,Sturdevant DE,Porcella SF,Doumbo OK,Doumbo S,Kayentao K,Ongoiba A,Traore B,Sanz I,Pierce SK,Crompton PD2.Journal of immunology (Baltimore, Md. : 1950)Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.2013"45-0198 was used in Flow cytometry/Cell sorting to study B and T cell exhaustion caused by chronic exposure to Plasmodium falciparum."Authors Illingworth J,Butler NS,Roetynck S,Mwacharo J,Pierce SK,Bejon P,Crompton PD,Marsh K,Ndungu FM3.European journal of immunologyLong-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.2013"45-0198 was used in Flow cytometry/Cell sorting to expose long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers."Authors Ndungu FM,Lundblom K,Rono J,Illingworth J,Eriksson S,Färnert A4.Malaria journalB cell analysis of ethnic groups in Mali with differential susceptibility to malaria."45-0198 was used in Flow cytometry/Cell sorting to determine the mechanisms underlying the elevated resistance to malaria observed in the Fulani ethnic group."2012Authors Portugal S,Doumtabe D,Traore B,Miller LH,Troye-Blomberg M,Doumbo OK,Dolo A,Pierce SK,Crompton PD |
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