ANTI-HUMAN CD38 (HIT2) PE-eFluor 610
货号：61-0389-41,61-0389-42
规格：25TESTS,100TESTS
价格：0,3035
产品类型：流式抗体
品牌：eBioscience
抗原：CD38
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：HIT2
荧光染料：PE-eFluor 610
| 抗体类型: | 单抗 | 同型对照： | IgG1, kappa |
| 免疫原性： | | 用法： | 5uL（0.125µg）/test(Flow） |
| Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.Applications Reported: This HIT2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD38 Antibody (61-0389-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-eFluor® 610 (Product # 61-4714-82) (blue histogram) or Anti-Human CD38 PE-eFluor® 610 (purple histogram). Cells in the lymphocyte (left) and monocyte (right) gates were used for analysis. |
| 推荐产品： |
| 造血干细胞HSC分选造血干细胞HSC扩增、分化培养基CD38流式抗体细胞因子 |
| 参考文献： |
| 1.The Biochemical journalCD38 binding to human myeloid cells is mediated by mouse and human CD31.Authors Horenstein AL,Stockinger H,Imhof BA,Malavasi F |
技术参数 产品应用 Flow；MISC
细胞类型 HSC
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