ANTI-HUMAN CD45RA (JS-83) PE
货号：12-9979-41,12-9979-42
规格：25TESTS,100TESTS
价格：720.75,2142
产品类型：流式抗体
品牌：eBioscience
抗原：CD45RA
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：JS-83
荧光染料：PE
| 抗体类型: | 单抗 | 同型对照： | IgG1, kappa |
| 免疫原性： | | 用法： | 5uL（10.03µg）/test(Flow） |
| Description: The JS-83 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4^+ peripheral T lymphocytes, CD8^+ peripheral T lymphocytes, and B cells. The CD45RA^+ T cell populations are mainly naive/virgin allowing the use of JS-83 mAb as a phenotypic marker to discriminate T cell subsets.Applications Reported: JS-83 has been reported for use in flow cytometric analysis.Applications Tested: This JS-83 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
| CD45RA Antibody (12-9979-41) in FlowSurface staining of normal human peripheral blood cells with Anti-Human CD45RA FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis. |
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| 参考文献： |
| 1.BMC infectious diseasesHuman immunodeficiency virus type I-specific CD8+ T cell subset abnormalities in chronic infection persist through effective antiretroviral therapy."12-9979 was used in Flow cytometry/Cell sorting to evaluate the magnitude, general specificity and character of HIV specific CD8+ T cell responses, showing CD8+ T cell abnormalities in chronic infection."Authors Pohling J,Zipperlen K,Hollett NA,Gallant ME,Grant MD2.Molecular immunologyTCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses."12-9979 was used in Flow cytometry/Cell sorting to investigate how PTP-PEST is down-regulated following T cell activation, and how that can lead to reduced IL-2 secretion following TCR and CD28 stimulation."Authors Arimura Y,Vang T,Tautz L,Williams S,Mustelin T |
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