CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), Functional Grade, eBioscience™
货号：16-0291-85
规格：500 µg
价格：3982
产品类型：流式抗体
品牌：eBioscience
抗原：CD29
物种：人/小鼠/大鼠
宿主：Armenian hamster仓鼠
抗体亚型：IgG
克隆号：eBioHMb1-1 (HMb1-1)
荧光染料：其它
| 抗体类型: | 单抗 | 同型对照： | IgG |
| 免疫原性： | | 用法： | 1 µg/test(Flow） |
Description: The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110-120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f to form the VLA-1 through VLA-6 complexes, as well as with CD51. These alpha-beta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins can also interact with cellular receptors such as VCAM-1 and MadCAM-1.Applications Reported: This eBioHMb1-1 (HMb1-1) antibody has been reported for use in flow cytometric analysis. The HMb1-1 monoclonal antibody has been reported to block VLA-dependent cellular functions, including the adhesion of mouse tumor cell lines to extracellular matrix proteins, and splenic T-cell proliferation induced by anti-CD3 monoclonal antibody.Applications Tested: This eBioHMb1-1 (HMb1-1) antibody has been tested by flow cytometric analysis of mouse spleen, thymus and bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.Background/Target InformationCD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
| 数据 |
| CD29 (Integrin beta 1) Antibody (16-0291-85) in FlowStaining of C57Bl/6 thymocytes with 0.5 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888) (open histogram) or 0.5 µg of Anti-Mouse/Rat CD29 Purified (filled histogram) followed by Anti-Armenian Hamster IgG Biotin (Product # 13-4113) and Streptavidin PE(Product # 12-4317).Total viable cells were used for analysis. |
| 推荐产品： |
| 间充干细胞MSC分选间充干细胞MSC扩增、分化培养基CD29流式抗体细胞因子 |
| 参考文献： |
| 1.The Journal of clinical investigationFOXP1 controls mesenchymal stem cell commitment and senescence during skeletal aging.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer2.PloS oneMicrogrooved-surface topography enhances cellular division and proliferation of mouse bone marrow-derived mesenchymal stem cells.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer3.Molecular therapy oncolyticsPreclinical evaluation of VAX-IP, a novel bacterial minicell-based biopharmaceutical for nonmuscle invasive bladder cancer.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer4.Experimental and therapeutic medicineTreatment of radiation-induced acute intestinal injury with bone marrow-derived mesenchymal stem cells.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer5.Proceedings of the National Academy of Sciences of the United States of AmericaCasitas B-cell lymphoma (Cbl) proteins protect mammary epithelial cells from proteotoxicity of active c-Src accumulation.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-16791.htm
QQ:1749072012