CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), eBioscience™

货号：14-0113-81

规格：50μg

价格：1156

产品类型：抗体和ELISA

品牌：eBioscience

抗体类型:	单抗	同型对照：	IgG1, kappa
浓度：	0.5 mg/mL	用法：	1 µg/test(Flow)；Assay-Dependent(FN)；Assay-Dependent(IP)；Assay-Dependent(Neu)

Description: The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the alpha chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1). It should be noted that low level activation may occur during processing of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1 in these unstimulated samples. However, higher levels of Mac-1 expression are observed in activated samples when compared to unstimulated cells.Applications Reported: The CBRM1/5 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. CBRM1/5 has also been reported in blocking of CD11b function. (Please use Functional Grade purified CBRM1/5, cat. 16-0113, in functional assays.).Applications Tested: The CBRM1/5 antibody has been tested by flow cytometric analysis of resting and activated normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

CD11b (activation epitope) Antibody (14-0113-81)Arteriosclerosis, thrombosis, and vascular biology 2018 -Figure 5. FAM3D (family with sequence similarity 3, member D) induces neutrophil adhesion and transmigration and activates Mac-1 (macrophage-1 antigen) in neutrophils. A , Representative photographs and quantification of the adhesion of DiI-labeled neutrophils to human umbilical vein endothelial cells (HUVECs) after coculture in the absence or presence of FAM3D neutralization antibody 6D7 (20 mumol/L) or an equal amount of mouse IgG for 2 hours. HUVEC monolayers were stimulated with 4 ng/mL TNF-alpha (tumor necrosis factor alpha) for 24 hours prior to coculture. n=12. One-way analysis of variance (ANOVA) followed by Tukey's test for multiple comparisons, * P <0.05. B , Representative intravital microscopic photographs and quantification of leukocyte rolling and adhesion to mesentery microvessels of wild-type (WT; n=8) and FAM3D -/- (n=8) mice 4 hours after intraperitoneal injection with or without TNF-alpha (500 ng for each mice). Two-way ANOVA followed by Tukey's test for multiple comparisons, * P <0.05, ns, no significance. C , Representative photographs and quantification of DiI-stained mouse neutrophils transmigrated through a confluent layer of HUVECs in response to 4 hours of treatment with mouse FAM3D. n=12. One-way ANOVA followed by Tukey's test for multiple comparisons, * P <0.05. D , Real-time polymerase chain reaction (PCR) quantification of mRNA levels of Mac-1 (CD11b) in mouse neutrophils stimulated by FAM3D for 4 hours. beta-Actin was applied as an interna

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参考文献：

1. Nature communicationsLECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells."14011381 was used in flow cytometry to find that leukocyte cell-derived chemotaxin 2 promotes expansion and mobilization of hematopoietic stem cells"AuthorsLu XJ,Chen Q,Rong YJ,Yang GJ,Li CH,Xu NY,Yu CH,Wang HY,Zhang S,Shi YH,Chen J2. The Journal of pharmacology and experimental therapeuticsAnnexin A1 mediates hydrogen sulfide properties in the control of inflammation."14-0113 was used in Flow cytometry/Cell sorting to investigate whether endogenous annexin A1 could display intermediary functions in the anti-inflammatory profile of hydrogen sulfide."AuthorsBrancaleone V,Mitidieri E,Flower RJ,Cirino G,Perretti M3. Arthritis and rheumatismMultiple lupus-associated ITGAM variants alter Mac-1 functions on neutrophils."14-0113 was used in Flow cytometry/Cell sorting to determine the effects of ITGAM genetic variation on the functions of neutrophil Mac-1."AuthorsZhou Y,Wu J,Kucik DF,White NB,Redden DT,Szalai AJ,Bullard DC,Edberg JC4. BloodDasatinib inhibits proinflammatory functions of mature human neutrophils."14-0113 was used in Flow cytometry/Cell sorting to suggest that dasatinib treatment may affect the proinflammatory functions of mature neutrophils."AuthorsFutosi K,Németh T,Pick R,Vántus T,Walzog B,Mócsai A5. BloodAntiadhesive effect of fibrinogen: a safeguard for thrombus stability."14-0113 was used in Flow cytometry/Cell sorting to investigate whether soluble fibrinogen could protect fibrin from excessive adhesion of leukocytes."AuthorsLishko VK,Burke T,Ugarova T

技术参数

产品应用 Flow;FN;IP;Neu

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