CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), eFluor 450

货号：48-5971-80,48-5971-82

规格：25μg,100μg

价格：1038,3050

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgM

克隆号：DX5

荧光染料：eFluor 450

抗体类型:	单抗	同型对照：	Rat IgM Isotype Control, eFluor 450, eBioscience™
免疫原性：		用法：	1 µg/test(Flow）

Description: The DX5 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer cells and a subset of T cells. DX5 reacts with all strains of mouse tested including the most commonly used strains, BALB/c, C57BL/6, C3H, CBA, DBA, AKR, SJL and 129. Simultaneous staining of C57BL/6 spleen cells with anti-NK1.1 mAb (PK136) and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.Applications Reported: This DX5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This DX5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationGTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Gi belongs to the pertussis toxin sensitive G-proteins. Gi consists of three different subtypes of a-subunits (ai1, ai2 and ai3).For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD49b (Integrin alpha 2) Antibody (48-5971-80) in Flow Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and 0.5 µg of Anti-Mouse CD49b(Integrin alpha 2) eFluor® 450. Total viable cells were used for analysis.

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参考文献：

1. PloS oneDifferential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase."48-5971 was used in Flow cytometry/Cell sorting to demonstrate a complex regulation pattern of C5aR1 in the airways, lung tissue and lymph nodes of mice."AuthorsEnder F,Wiese AV,Schmudde I,Sun J,Vollbrandt T,König P,Laumonnier Y,Köhl J2. The Journal of experimental medicineDC subset-specific induction of T cell responses upon antigen uptake via Fcγ receptors in vivo."48-5971 was used in Flow cytometry/Cell sorting to suggest that targeting antigens to FcγRs on DCs may be a promising approach to raise immunogenic or tolerogenic T cell responses to antigens of choice."AuthorsLehmann CHK,Baranska A,Heidkamp GF,Heger L,Neubert K,Lühr JJ,Hoffmann A,Reimer KC,Brückner C,Beck S,Seeling M,Kießling M,Soulat D,Krug AB,Ravetch JV,Leusen JHW,Nimmerjahn F,Dudziak D3. Journal of immunology (Baltimore, Md. : 1950)VprBP Is Required for Efficient Editing and Selection of Igκ+ B Cells, but Is Dispensable for Igλ+ and Marginal Zone B Cell Maturation and Selection."48-5971 was used in Flow cytometry/Cell sorting to determine the contribution of the protein, VprBP, to B cell development and selection."AuthorsPalmer VL,Aziz-Seible R,Kassmeier MD,Rothermund M,Perry GA,Swanson PC4. Journal of immunology (Baltimore, Md. : 1950)Monitoring and cell-specific deletion of C5aR1 using a novel floxed GFP-C5aR1 reporter knock-in mouse."48-5971 was used in Flow cytometry/Cell sorting to study cell-specific deletion of C5aR1 using a GFP-reporter knock-in mouse model."AuthorsKarsten CM,Laumonnier Y,Eurich B,Ender F,Bröker K,Roy S,Czabanska A,Vollbrandt T,Figge J,Köhl J5. Clinical and experimental immunologyA gluten-free diet lowers NKG2D and ligand expression in BALB/c and non-obese diabetic (NOD) mice."48-5971 was used in Flow cytometry/Cell sorting to show that a gluten-free diet reduces the level of NKG2D and the expression of NKG2D ligands."AuthorsAdlercreutz EH,Weile C,Larsen J,Engkilde K,Agardh D,Buschard K,Antvorskov JC

技术参数

产品应用 Flow

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