Rat IgG2a kappa Isotype Control, APC

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Rat IgG2a kappa Isotype Control, APC

货号:17-4321-81

规格:50μg

价格:1340

产品类型:大鼠同型对照

品牌:Thermo Fisher

物种:其它

宿主:大鼠

抗体亚型:IgG2a, kappa

克隆号:eBR2a

荧光染料:APC

抗体类型:

单抗

同型对照:

IgG2a, kappa

免疫原性:

用法:

Assay-Dependent (Ctrl);Assay-Dependent (Flow)

Description: The monoclonal rat IgG2a kappa is useful as an isotype control immunoglobulin.Applications Reported: This eBR2a antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBR2a antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells and should be used at the same concentration as the experimental antibody.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献:

1. European journal of medical researchThe inflammatory phase of fracture healing is influenced by oestrogen status in mice."17-4321 was used in Immunohistochemistry to indicate that oestrogen-deficiency influences the early inflammatory phase after fracture."AuthorsHaffner-Luntzer M,Fischer V,Prystaz K,Liedert A,Ignatius A2. Nature communicationsEndothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells."17-4321 was used in Flow cytometry/Cell sorting to identify a novel and unexpected population of progenitors for coronary mural cells with relevance to heart function."AuthorsChen Q,Zhang H,Liu Y,Adams S,Eilken H,Stehling M,Corada M,Dejana E,Zhou B,Adams RH3. Molecular therapy oncolyticsIntratumoral INF-γ triggers an antiviral state in GL261 tumor cells: a major hurdle to overcome for oncolytic vaccinia virus therapy of cancer."17-4321 was used in Flow cytometry/Cell sorting to identify microenvironmental factors, which may be decisive for failure or success of oncolytic VACV therapy."AuthorsKober C,Weibel S,Rohn S,Kirscher L,Szalay AA4. Frontiers in bioscience (Landmark edition)Caspase-1 mediates hyperlipidemia-weakened progenitor cell vessel repair."17-4321 was used in Flow cytometry/Cell sorting to provide insight on how hyperlipidemia activates caspase-1 in Sca-1+ progenitor cells, which subsequently weakens their repair of vasculature injury."AuthorsLi YF,Huang X,Li X,Gong R,Yin Y,Nelson J,Gao E,Zhang H,Hoffman NE,Houser SR,Madesh M,Tilley DG,Choi ET,Jiang X,Huang CX,Wang H,Yang XF5. Circulation researchCardiac Fibro-Adipocyte Progenitors Express Desmosome Proteins and Preferentially Differentiate to Adipocytes Upon Deletion of the Desmoplakin Gene."17-4321 was used in Flow cytometry/Cell sorting to demonstrate how a subset of cardiac fibro-adipocyte progenitors expresses desmosome proteins and differentiates to adipocytes in arrhythmogenic cardiomyopathy through a Wnt-dependent mechanism."AuthorsLombardi R,Chen SN,Ruggiero A,Gurha P,Czernuszewicz GZ,Willerson JT,Marian AJ
技术参数

产品应用 Flow;Ctrl

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