FOXP3 Monoclonal Antibody (FJK-16s), APC

货号：17-5773-80,17-5773-82

规格：25μg,100μg

价格：3163,4214

产品类型：流式抗体

品牌：Thermo Fisher

物种：大鼠

宿主：大鼠

抗体亚型：IgG

克隆号：FJK-16s

荧光染料：APC

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	1 µg/test(Flow）

Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.Please note that FJK-16s has been optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (cat. 00-5523). The use of other fixation and staining buffers is not recommended.Applications Reported: This FJK-16s antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This FJK-16s antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcrition Factor Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This antibody can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationFOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

FOXP3 Antibody (17-5773-82) in Flow Staining of mouse splenocytes with Anti-Mouse CD4 FITC (Product # 11-0041-82) (left) and Anti-Mouse CD25 eFluor® 450 (Product # 48-0251-82) (right) followed by intracellular with Anti-Mouse Foxp3 APC using Foxp3/Transcription Factor Staining Buffers (Product # 00-5523-00). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. PloS oneMultiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. The Journal of biological chemistryLoss of Functionally Redundant p38 Isoforms in T Cells Enhances Regulatory T Cell Induction.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. EBioMedicineDendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Scientific reportsPaeoniflorin Ameliorates Experimental Autoimmune Encephalomyelitis via Inhibition of Dendritic Cell Function and Th17 Cell Differentiation....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. Stem cell reportsMSC-derived Extracellular Vesicles Attenuate Immune Responses in Two Autoimmune Murine Models: Type 1 Diabetes and Uveoretinitis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

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