CD117 (c-Kit) Monoclonal Antibody (2B8), APC, eBioscience™

货号：17-1171-81,17-1171-82,17-1171-83

规格：50μg,100μg,200μg

价格：1632,2117,3789

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：2B8

荧光染料：APC

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	0.125 µg/test(Flow）

Description: The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor, and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145-kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1 low) and negative for lineage markers. The interaction of the mouse c-Kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells.Applications Reported: The 2B8 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 2B8 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target Informationc-Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1 and 2 signaling pathway.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD117 (c-Kit) Antibody (17-1171-81) in Flow Staining of C57Bl/6 bone marrow cells with Mouse Hematopoietic Lineage eFluor® 450 Cocktail (Product # 88-7772-72), Anti-Mouse Ly-6A/E (Sca-1) PerCP-Cyanine5-5 (Product # 45-5981-82) and 0.06 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (left) or 0.06 µg of Anti-Mouse CD117 (c-Kit) APC (right). Lineage negative/low, viable cells, as determined by Fixable Viability Dye eFluor® 780 (Product # 65-0865-14), were used for analysis.

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参考文献：

1. Scientific reportsTargeted genome editing restores T cell differentiation in a humanized X-SCID pluripotent stem cell disease model.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. eLifelncRNA requirements for mouse acute myeloid leukemia and normal differentiation."17-1171 was used in Flow cytometry/Cell sorting to assess lncRNA function via an in vivo RNAi screen in a model of acute myeloid leukemia."AuthorsDelás MJ,Sabin LR,Dolzhenko E,Knott SR,Munera Maravilla E,Jackson BT,Wild SA,Kovacevic T,Stork EM,Zhou M,Erard N,Lee E,Kelley DR,Roth M,Barbosa IA,Zuber J,Rinn JL,Smith AD,Hannon GJ3. Stem cell research and therapyAstaxanthin attenuates total body irradiation-induced hematopoietic system injury in mice via inhibition of oxidative stress and apoptosis."17-1171 was used in Flow cytometry/Cell sorting to suggest that ATX could be used as a potent therapeutic agent to protect the hematopoietic system against TBI-induced bone marrow suppression."AuthorsXue XL,Han XD,Li Y,Chu XF,Miao WM,Zhang JL,Fan SJ4. PloS oneMitochondrial-Targeted Decyl-Triphenylphosphonium Enhances 2-Deoxy-D-Glucose Mediated Oxidative Stress and Clonogenic Killing of Multiple Myeloma Cells."17-1171 was used in Flow cytometry/Cell sorting to a novel strategy of oxidative stress-induced cytotoxic killing of MM cells."AuthorsSchibler J,Tomanek-Chalkley AM,Reedy JL,Zhan F,Spitz DR,Schultz MK,Goel A5. Cell stem cellAdipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration."17-1171 was used in Flow cytometry/Cell sorting to specify the cellular identities of the adipogenic and osteogenic lineages of the bone."AuthorsAmbrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ

技术参数

产品应用 Flow

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