CD80 (B7-1) Monoclonal Antibody (16-10A1), APC

货号：17-0801-81,17-0801-82

规格：50μg,100μg

价格：1674,2788

产品类型：流式抗体

品牌：Thermo Fisher

物种：其它

宿主：仓鼠

抗体亚型：IgG

克隆号：16-10A1

荧光染料：APC

抗体类型:	单抗	同型对照：	Armenian Hamster IgG Isotype Control, APC, eBioscience™
免疫原性：		用法：	0.06 µg/test(Flow）

Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: The 16-10A1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD80 (B7-1) Antibody (17-0801-82) in FlowMouse splenocytes were unstimulated (left) or stimulated for 2 days with F (ab')2 Anti-Mouse IgM, u chain specific Functional Grade Purified (Product # 16-5092-85) and Anti-Mouse CD40 Functional Grade Purified (Product # 16-0401-82) (right). Cells were then stained with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.03 µg of Armenian Hamster IgG Isotype Control APC (Product # 17-4888-82) (blue histogram) or 0.03 µg of Anti-Mouse CD80 (B7-1) APC (purple histogram). Cell in the CD45R (B220) positive gate were used for analysis

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参考文献：

1.Molecular medicine reportsDiscrimination of the heterogeneity of bone marrow‑derived dendritic cells.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Frontiers in immunologyFcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Scientific reportsDer f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. EBioMedicineDendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. Scientific reportsPplase of Dermatophagoides farinae promotes ovalbumin-induced airway allergy by modulating the functions of dendritic cells in a mouse model....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

产品类型 Flow

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