CD40 Monoclonal Antibody (5C3), APC

货号：17-0409-41,17-0409-42

规格：25 Tests,100 Tersts

价格：1492,2886

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：5C3

荧光染料：APC

抗体类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	5uL（0.06µg）/test(Flow）

Description: The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.Applications Reported: This 5C3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 5C3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD40 Antibody (17-0409-41) in Flow Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (open histogram) or Anti-Human CD40 APC (filled histogram). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.OncotargetSurrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. BMC biotechnologyA simple, accurate and universal method for quantification of PCR.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Journal of virologyHepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism."17-0409 was used in Flow cytometry/Cell sorting to identify CD14 and TLR4 as receptors for HBsAg, the main HBV envelope antigen."Authorsvan Montfoort N,van der Aa E,van den Bosch A,Brouwers H,Vanwolleghem T,Janssen HLA,Javanbakht H,Buschow SI,Woltman AM4. Oncoimmunologymyeloid-derived suppressor cells are associated with progression of human NSCLC.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. PLoS pathogensNovel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses."17-0409 was used in Flow cytometry/Cell sorting to provide a previously unknown mechanism for parasite-induced subversion of adaptive immunity."AuthorsPreston SG,Majtán J,Kouremenou C,Rysnik O,Burger LF,Cabezas Cruz A,Chiong Guzman M,Nunn MA,Paesen GC,Nuttall PA,Austyn JM

技术参数

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