Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 790/驴抗兔 IgG (H+L) 高交叉吸附二抗，Alexa Fluor 790
货号：A11374
规格：500μl
价格：3545
产品类型：荧光二抗
品牌：Thermo Fisher
物种：兔
宿主：驴
抗体亚型：IgG
荧光染料：Alexa Fluor 790
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抗体类型: | 荧光二抗 | 同型对照： | IgG |
免疫原性： | Gamma Immunoglobins | 用法： | 1-10 µg/mL (ICC)；1-10 µg/mL (IF)； 0.04-0.2 µg/mL (WB) |
This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities. This antibody shows minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins.Background/Target InformationAnti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
数据 |
| Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A11374) in WBWestern blot analysis was performed on nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1) and U-87 MG (Lane 2). The blots were probed with ABfinity™ Anti-JUNB Recombinant Rabbit Monoclonal Antibody (Product # 701702, 2 µg/mL) and detected using Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 790 (Product # A11374) at concentrations 0.04 µg/mL (Fig. 1), 0.1 µg/mL (Fig. 2) and 0.2 µg/mL (Fig. 3). A 42 kDa band corresponding to JUNB was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences). |
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参考文献： |
| 1. Cellular logisticsPlasmids for variable expression of proteins targeted to the mitochondrial matrix or intermembrane space."A11374 was used in western blot to develop plasmids to study mitochondrial proteins"AuthorsNewman LE,Schiavon C,Kahn RA2. Cancer scienceIncreasing circulating exosomes-carrying TRPC5 predicts chemoresistance in metastatic breast cancer patients."A11374 was used in western blot to explore the contribution of TRPC5 carrying exosomes to breast cancer"AuthorsWang T,Ning K,Lu TX,Sun X,Jin L,Qi X,Jin J,Hua D3. Oncology reportsB7-H3 upregulates BRCC3 expression, antagonizing DNA damage caused by 5-Fu."A11374 was used in western blot to suggest that BRCC3 has a role in B7-H3-induced 5-fluorouracil resistance"AuthorsSun ZZ,Zhang T,Ning K,Zhu R,Liu F,Tang SC,Jiang B,Hua D |
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