Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 680/羊抗兔 IgG (H+L) 交叉吸附荧光二抗, Alexa Fluor 680

货号：A-21096

规格：500μl

价格：3528

产品类型：荧光二抗

品牌：Thermo Fisher

物种：大鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 680

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	1-10 μg/mL (ICC)；1-10 μg/mL (IF)； 0.04-0.2 μg/mL (WB)

This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.Background/Target InformationAnti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rat IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21096) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1) and NCCIT (Lane 2). The blots were probed with Anti-alpha Tubulin Rat Monoclonal Antibody (Product # MA1-80017, 1 µg/mL) and detected using Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 680 (Product # A-21096) at dilutions 0.04 µg/mL (Fig. 1), 0.1 µg/mL (Fig. 2) and 0.2 µg/mL (Fig. 3). A 52 kDa band corresponding to alpha Tubulin was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences).

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参考文献：

1. Genes and developmentAcute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a."A21096 was used in flow cytometry to explore the role of Hhex in acute myeloid leukemia"AuthorsShields BJ,Jackson JT,Metcalf D,Shi W,Huang Q,Garnham AL,Glaser SP,Beck D,Pimanda JE,Bogue CW,Smyth GK,Alexander WS,McCormack MP2. Journal of immunology (Baltimore, Md. : 1950)The adaptor protein Bam32 in human dendritic cells participates in the regulation of MHC class I-induced CD8+ T cell activation.AuthorsOrtner D,Grabher D,Hermann M,Kremmer E,Hofer S,Heufler C3. The Journal of biological chemistryThe phospholipid-binding protein SESTD1 is a novel regulator of the transient receptor potential channels TRPC4 and TRPC5.AuthorsMiehe S,Bieberstein A,Arnould I,Ihdene O,Rütten H,Strübing C4. Molecular biology of the cellNascent transcription of MCM2-7 is important for nuclear localization of the minichromosome maintenance complex in G1.AuthorsBraun KA,Breeden LL

技术参数

产品应用 ICC;IF;WB

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