Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 635/羊抗兔 IgG (H+L) 高交叉吸附 荧光二抗,Alexa Fluor 635

货号：A-31577

规格：500μl

价格：3719

产品类型：荧光二抗

品牌：Thermo Fisher

物种：兔

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 635

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	1-10 μg/mL（IHC）；4 μg/mL (ICC)；4 μg/mL (IF）

Background/Target InformationAnti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-31577) in IFImmunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 635 was performed using HepG2 cells stained with alpha-1 antitrypsin Rabbit Polyclonal Primary Antibody (Product # PA5-16661). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Rabbit primary antibody (1:250 dilution) 3 hours at room temperature. Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 635 (Product # A-31577) was used at a concentration of 4 µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha-1 antitrypsin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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参考文献：

1. Brain structure and functionUsing a novel PV-Cre rat model to characterize pallidonigral cells and their terminations."A31577 was used in immunohistochemistry - frozen section to perform morphological and electrophysiological investigations of axons from parvalbumin-Cre rat neurons in globus pallidus"AuthorsOh YM,Karube F,Takahashi S,Kobayashi K,Takada M,Uchigashima M,Watanabe M,Nishizawa K,Kobayashi K,Fujiyama F2．Development (Cambridge, England)Induced stem cell neoplasia in a cnidarian by ectopic expression of a POU domain transcription factor.AuthorsMillane RC,Kanska J,Duffy DJ,Seoighe C,Cunningham S,Plickert G,Frank U3. Development (Cambridge, England)Msx genes define a population of mural cell precursors required for head blood vessel maturation.AuthorsLopes M,Goupille O,Saint Cloment C,Lallemand Y,Cumano A,Robert B4. American journal of physiology. Endocrinology and metabolismRole for cumulus cell-produced EGF-like ligands during primate oocyte maturation in vitro.AuthorsNyholt de Prada JK,Lee YS,Latham KE,Chaffin CL,VandeVoort CA5. The Journal of biological chemistryRhoA GTPase and F-actin dynamically regulate the permeability of Cx43-made channels in rat cardiac myocytes.AuthorsDerangeon M,Bourmeyster N,Plaisance I,Pinet-Charvet C,Chen Q,Duthe F,Popoff MR,Sarrouilhe D,Hervé JC

技术参数

产品应用 ICC;IF;IHC

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