GST Tag Monoclonal Antibody (8-326), Biotin/GST标签抗体

货号：MA4-004-BTIN

规格：50 µL

价格：2753

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：Biotin

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	IgG1
免疫原性：	Recombinant purified full-length GST	用法：	1:500-1:1000(WB)

Product Specific InformationMA4-004-BTIN has been successfully for Western blot and IP.Background/Target InformationGST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

GST Tag Antibody (MA4-004-BTIN) in WBWestern blot analysis of GST was performed by loading various amounts of purified GST-SRC SH2 domain per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a biotinylated GST monoclonal antibody (Product # MA4-004-BTIN) at a dilution of 1:1000 for 1 hour at room temperature, followed by Streptavidin-HRP (Product # 21126) at a dilution of 1:20,000 for 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078). GST Tag Antibody (MA4-004-BTIN) in WBImmuno-precipitation of GST-Shp2 SH2 domain (Product # 87709) (Lane 2, 39 kDa) was performed by mixing 2 µg of purified fusion protein with1 µg of mouse monoclonal antibody recognizing GST (Product # MA4-004) overnight on a rocking platform at 4°C. The immune-complex was then captured on 50 µL Protein A/G Plus Agarose (Product # 20423). Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye (Product # 39000). Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. IP efficiency was compared to a non-IP loading control consisting of 1 µg GST-Shp2 SH2 domain (Lane1). Proteins were transferred to PVDF membrane and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing GST (Product # MA4-004) at a dilution of 1:1000 overnight rotating at 4°C. Membranes were then washed in TBS/0.1% Tween-20 and probed with Clean-blot IP detection reagent (Product # 21230) at a dilution of 1:2000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Dura (Product # 34075). GST Tag Antibody (MA4-004-BTIN) in IPWestern blot analysis of GST-Abl SH2 domain (Lane 1, 35 kDa) (Product # 87702) and GST-Grb2 (Lane 2, 38 kDa) SH2 domain (Product # 87700) was performed by loading 2 µg of each purified fusion protein onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/0.1%TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing GST clone 8-826 (Product # MA4-004) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS/0.1%Tween-20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:25000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce SuperSignal West Dura (Product # 34075).

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参考文献：

1.Oncology letters A potential peptide vector that allows targeted delivery of a desired fusion protein into the human breast cancer cell line MDA-MB-231. View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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