GFP Monoclonal Antibody (GF28R), DyLight 680

货号：MA5-15256-D680

规格：50 µL

价格：2753

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：DyLight 680

抗体类型:	单抗	同型对照：	IgG1
免疫原性：	GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugated.	用法：	1:25-100（ICC）；1:25-100（IF）；1:500-1:1000(WB)

Product Specific InformationMA5-15256-D680 has been successfully used in immunofluorescence and Western blot applications. DyLight 680 has an excitation/emission of 692/712 nm.Background/Target InformationThe jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organinsms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcelluclar compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used etensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

GFP Antibody (MA5-15256-D680) in WBWestern blot analysis of GFP was performed by loading various amounts of recombinant GFP per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated GFP monoclonal antibody (Product # MA5-15256-D680) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey. GFP Antibody (MA5-15256-D680) in IFImmunofluorescent analysis of HeLa cells transfected with a construct containing a GFP Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 680-conjugated GFP Tag monoclonal antibody (Product # MA5-15256-D680) at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.

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参考文献：

1.PloS oneREGULATOR OF BULB BIOGENESIS1 (RBB1) Is Involved in Vacuole Bulb Formation in Arabidopsis.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2.Nature chemical biologyTunable and reversible drug control of protein production via a self-excising degron.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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