V5 Tag Monoclonal Antibody (E10/V4RR)/V5标签抗体

货号：MA5-15253,MA5-15253-1MG

规格：100 ug,1 mg

价格：2753,14506

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：其它

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	IgG1
免疫原性：	GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH	用法：	1:500-1:2000（IF）；1:1000-1:3000(WB)

Product Specific InformationMA5-15253 recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins.MA5-15253 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.Background/Target InformationEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST),although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

V5 Tag Antibody (MA5-15253) in WB1:1000 (1 µg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2) V5 Tag Antibody (MA5-15253) in WBWestern blot analysis of V5-tagged proteins was performed by loading 15 µg of 293T cell lysates, from cells transfected with a V5-Wnt3a or V5-Vstm2 construct for the indicated number of hours, per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% milk in PBST for 1 hour at room temperature. The membrane was probed with a V5 Epitope Tag monoclonal antibody (Product # MA5-15253) at a dilution of 1:1000 overnight at 4°C, washed in PBST, and probed with an HRP-conjugated anti-mouse IgG secondary antibody at a dilution of 1:5000 for 1 hour at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.

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参考文献：

1.PLoS pathogensTRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP)."MA515253 was used in western blot to investigate the antiviral mechanism of host factor and interferon-stimulated gene product, zinc-finger antiviral protein"AuthorsLi MM,Lau Z,Cheung P,Aguilar EG,Schneider WM,Bozzacco L,Molina H,Buehler E,Takaoka A,Rice CM,Felsenfeld DP,MacDonald MR2.Journal of molecular biologySelective Targeting of SH2 Domain-Phosphotyrosine Interactions of Src Family Tyrosine Kinases with Monobodies."MA515253 was used in flow cytometry to target Src family kinase Src-homology 2 domains with high selectivity via the development of synthetic binding proteins, termed monobodies"AuthorsKükenshöner T,Schmit NE,Bouda E,Sha F,Pojer F,Koide A,Seeliger M,Koide S,Hantschel O3.The Journal of biological chemistryChemical-controlled Activation of Antiviral Myxovirus Resistance Protein 1."MA515253 was used in immunoprecipitation to characterize a mouse antiviral myxovirus resistance protein 1 variant that only exerts antiviral activity in the presence of a small molecule drug"AuthorsVerhelst J,Van Hoecke L,Spitaels J,De Vlieger D,Kolpe A,Saelens X4.Developmental cellPrecise Editing of the Zebrafish Genome Made Simple and Efficient."MA5-15253 was used in Immunohistochemistry to significantly advance applicability of the zebrafish for studying normal biological processes and modeling diseases."AuthorsHoshijima K,Jurynec MJ,Grunwald DJ5.Cell reportsTead and AP1 Coordinate Transcription and Motility."MA5-15253 was used in immunoprecipitation and western blot investigate Tead4-mediated transcriptional networks in various types of cancer cells"AuthorsLiu X,Li H,Rajurkar M,Li Q,Cotton JL,Ou J,Zhu LJ,Goel HL,Mercurio AM,Park JS,Davis RJ,Mao J

技术参数

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